RT Journal Article ID 2abbda07422a14f5 A1 Subramaniam, Sarasvathy A1 Raman, Jegadeesh A1 Sabaratnam, Vikineswary A1 Heng, Chua Kek A1 Kuppusamy, Umah Rani T1 Functional Properties of Partially Characterized Polysaccharide from the Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2017-11-29 VO 19 IS 10 SP 849 OP 859 K1 antiglycemic K1 antioxidant K1 Ganoderma neo-japonicum K1 medicinal mushrooms K1 polysaccharide AB This study was conducted to evaluate the mycochemical composition and antiglycemic and antioxidant activities of Ganoderma neo-japonicum hot aqueous extracts, prepared at different boiling durations, and polysaccharides isolated from them. Ground basidiocarps of G. neo-japonicum were double-boiled at 100°C for 0.5, 3, or 4 hours, and the antiglycemic activity was assessed by α-amylase and α-glucosidase enzyme inhibition assays. The antioxidant capacity of the crude hot aqueous extracts (AE-1, AE-2, AE-3) was assessed by DPPH and ABTS radical scavenging and ferric-reducing antioxidant power assays. The total phenolics, protein, and sugar in the crude extracts were also determined. The hot aqueous extract (AE-3) containing a significant amount of total sugar and having enhanced antiglycemic and antioxidant activities was selected for polysaccharide isolation. The isolated crude polysaccharide was separated and purified using diethylaminoethyl-cellulose-52 and Sepharose 6B column chromatography. Fourier transform infrared spectroscopy studies of the purified polysaccharide fraction (PF) showed the presence of typical bands corresponding to polysaccharides. The estimated β-glucan concentration in the PF was 39.26%. In general, the PF exhibited significantly lower antioxidant activity than AE-3. Nevertheless, its potency in inhibiting carbohydratehydrolyzing enzymes may have potential in the management of diabetes mellitus. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,305c54e9703680c8,2abbda07422a14f5.html