RT Journal Article ID 1ed5215d4bef89c8 A1 Cai, Ming A1 Lin, Yang A1 Luo, Yin-long A1 Liang, Han-hua A1 Sun, Peilong T1 Extraction, Antimicrobial, and Antioxidant Activities of Crude Polysaccharides from the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Higher Basidiomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2015-06-30 VO 17 IS 6 SP 591 OP 600 K1 medicinal mushrooms K1 Auricularia auricula-judae K1 crude polysaccharides K1 antimicrobial K1 antioxidant AB In this study, crude polysaccharides of culinary-medicinal mushroom Auricularia auricular-judae were extracted by hot water extraction and alcohol precipitation, and their antimicrobial and antioxidant activities were investigated. An optimum extraction condition was obtained at a ratio of liquid to solid 70 mL/g, temperature 90°C, time 4 h and extraction number 4. Accordingly, the best yield of crude polysaccharides was 6.89% with 76.12% in purity. Some bacteria and fungi were used for antimicrobial studies. It was found that crude A. auricula-judae had great antimicrobial activities against Escherichia coli and Staphylococcus aureus, but no activities on the others. The inhibitory diameters of antimicrobial zones for the two were 5.55 ± 0.182 and 9.84 ± 0.076 mm, respectively. Moreover, crude A. auricula-judae had significant antioxidant activities in scavenging free radicals, reducing power assays, and Fe2+ chelating ability assay. Results revealed that crude A. auricula-judae has a great potential as antimicrobial and antioxidant, and it can be a supplementary food for human health. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,52f67ca6221cc3fa,1ed5215d4bef89c8.html