RT Journal Article ID 17d31937050a7002 A1 Kumaran, Sekar A1 Pandurangan, Ashok Kumar A1 Shenbhagaraman, Ramalingam A1 Esa, Norhaizan Mohd. T1 Isolation and Characterization of Lectin from the Artist's Conk Medicinal Mushroom, Ganoderma applanatum (Agaricomycetes), and Evaluation of Its Antiproliferative Activity in HT-29 Colon Cancer Cells JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2017-10-16 VO 19 IS 8 SP 675 OP 684 K1 extracellular polysaccharides K1 immunostimulation K1 medicinal mushrooms K1 NK cell activity K1 phagocytic assay K1 splenocyte proliferation AB The growth and lectin production of Ganoderma applanatum, a white rot fungus, was optimized in broth cultures. The fungus was found to have a higher growth rate and higher lectin activity when grown in a medium adjusted to pH 6.5 at 26°C under stationary conditions. Expression of lectin activity started in 5-day-old mycelial culture; maximum activity was expressed after the 15th day of incubation. Among the various carbon and nitrogen sources tested, the carbon source sucrose and the nitrogen source yeast extract support maximum growth and lectin production. Lectin from G. applanatum was purified by ammonium sulfate precipitation and ion exchange chromatography. The purified fraction revealed a single band with a molecular weight of 35.0 kDa. Moreover, carbohydrates such as mannitol, glucose, sucrose, maltose, mannose, galactose, sorbose, and fructose were found to inhibit the hemagglutinating activity of the lectin. The purified lectins from G. applanatum contain cytotoxic and proapoptotic activities against HT-29 colon adenocarcinoma cells. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,32c8651274405055,17d31937050a7002.html