RT Journal Article
ID 0ff468ba53f74ef8
A1 Puchkova, Tatiana A.
A1 Shcherba, Victor V.
A1 Babitskaya, Valentina G.
T1 Comparative Examination of Polysaccharide Synthesis by Medicinal Mushrooms from the Genus Lentinus Fr. (Agaricomycetideae)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2010
FD 2010-05-26
VO 12
IS 2
SP 123
OP 132
K1 medicinal mushrooms
K1 Lentinus edodes
K1 Lentinus lepideus
K1 Lentinus tigrinus
K1 polysaccharides
K1 mycelium
K1 submerged cultivation
K1 cultivation conditions
AB The effect of culture conditions on growth and polysaccharide production by Lentinus edodes, L. lepideus, and L. tigrinus was investigated. These fungi accumulated 11.2−14.0 g/L biomass, 2.0−5.0 g/L exopolysaccharides, and 4.0%−10.2% endopolysaccharides on optimal glucose-peptone media. The maximum amount of endopolysaccharides (9.5%−10.2%) was found in L. lepideus biomass, whereas the highest level of exopolysaccharide synthesis (4.5−5.0 g/L) was observed in L. edodes. The specific growth rate of the fungi (μ) equaled 0.010 g/L for L. edodes, 0.020 g/L for L. lepideus, and 0.025 g/L for L. tigrinus; productivity of exopolysaccharide synthesis: 550, 300, and 500 mg/L per day, respectively; and productivity of endopolysaccharide synthesis: 40, 170, and 140 mg/L per day respectively. Fungal polysaccharides combined both high-molecular-weight fractions (Mr = 200−2000 kDa) and low-molecular-weight fractions (Mr < 10 kDa). As to carbohydrate composition, polysaccharides of investigated fungi were identified as heteroglycans, with glucose as the dominant monomer and minor amounts of arabinose, galactose, xylose, and mannose. For physical-chemical properties, a close similarity was shown between the polysaccharides of L. tigrinus and L. edodes.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,40aa21637478b2ab,0ff468ba53f74ef8.html