RT Journal Article ID 0ff468ba53f74ef8 A1 Puchkova, Tatiana A. A1 Shcherba, Victor V. A1 Babitskaya, Valentina G. T1 Comparative Examination of Polysaccharide Synthesis by Medicinal Mushrooms from the Genus Lentinus Fr. (Agaricomycetideae) JF International Journal of Medicinal Mushrooms JO IJM YR 2010 FD 2010-05-26 VO 12 IS 2 SP 123 OP 132 K1 medicinal mushrooms K1 Lentinus edodes K1 Lentinus lepideus K1 Lentinus tigrinus K1 polysaccharides K1 mycelium K1 submerged cultivation K1 cultivation conditions AB The effect of culture conditions on growth and polysaccharide production by Lentinus edodes, L. lepideus, and L. tigrinus was investigated. These fungi accumulated 11.2−14.0 g/L biomass, 2.0−5.0 g/L exopolysaccharides, and 4.0%−10.2% endopolysaccharides on optimal glucose-peptone media. The maximum amount of endopolysaccharides (9.5%−10.2%) was found in L. lepideus biomass, whereas the highest level of exopolysaccharide synthesis (4.5−5.0 g/L) was observed in L. edodes. The specific growth rate of the fungi (μ) equaled 0.010 g/L for L. edodes, 0.020 g/L for L. lepideus, and 0.025 g/L for L. tigrinus; productivity of exopolysaccharide synthesis: 550, 300, and 500 mg/L per day, respectively; and productivity of endopolysaccharide synthesis: 40, 170, and 140 mg/L per day respectively. Fungal polysaccharides combined both high-molecular-weight fractions (Mr = 200−2000 kDa) and low-molecular-weight fractions (Mr < 10 kDa). As to carbohydrate composition, polysaccharides of investigated fungi were identified as heteroglycans, with glucose as the dominant monomer and minor amounts of arabinose, galactose, xylose, and mannose. For physical-chemical properties, a close similarity was shown between the polysaccharides of L. tigrinus and L. edodes. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,40aa21637478b2ab,0ff468ba53f74ef8.html