年間 12 号発行
ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344
Indexed in
Partial Purification and Characterization of Polyphenoloxidase from Culinary-Medicinal Royal Sun Mushroom (the Himematsutake), Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae)
要約
The Royal Sun mushroom, the Himematsutake culinary-medicinal mushroom, Agaricus brasiliensis has several polyphenoloxidase activities in a broad sense. Here we report the partial purification of tyrosinase-type polyphenoloxidase (PPO). PPO is purified from A. brasiliensis without browning using a two-phase partitioning with Triton X-114 and ammonium sulfate fractionation. Partially denaturing SDS-PAGE (sodium dodecyl sulfate-polyacrylamide electrophoresis) staining with L-3,4-dihydroxyphenylalanine was performed and the indicated molecular sizes were approximately 70 kDa and 45 kDa. The purified enzyme is in its latent state and can be activated maximally in the presence of 1.6 mM sodium dodecyl sulfate (SDS). This enzyme catalyzes two distinct reactions, monophenolase and diphenolase activity, and the monophenolase activity showed a lag time typical of polyphenoloxidase. The Km value for 4-tert-butylcatechol was quite similar in the presence and absence of SDS, but the apparent Vmax value was increased 2.0-fold by SDS. Mimosine was a typical competitive inhibitor with Ki values of 138.2 μM and 281.0 μM n the presence and absence of SDS, respectively.
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Yamanaka Daisuke, Tada Rui, Adachi Yoshiyuki, Ishibashi Ken-ichi, Motoi Masuro, Iwakura Yoichiro, Ohno Naohito, Agaricus brasiliensis-derived β-glucans exert immunoenhancing effects via a dectin-1-dependent pathway, International Immunopharmacology, 14, 3, 2012. Crossref
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Mathur Ritika, Navya P. N., Basavaraj K., Murthy Pushpa S., Bioprocess of robusta cherry coffee with polyphenol oxidase and quality enhancement, European Food Research and Technology, 240, 2, 2015. Crossref
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Yamanaka Daisuke, Tamiya Yumi, Motoi Masuro, Ishibashi Ken-ichi, Miura Noriko N., Adachi Yoshiyuki, Ohno Naohito, Heimesaat Markus M., The Effect of Enzymatically Polymerised Polyphenols on CD4 Binding and Cytokine Production in Murine Splenocytes, PLoS ONE, 7, 4, 2012. Crossref