ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i5.30
pages 405-418

Tiger's Milk Medicinal Mushroom, Lignosus rhinocerotis (Agaricomycetes) Sclerotium Inhibits Nitric Oxide Production in LPS-Stimulated BV2 Microglia

Syntyche Ling-Sing Seow
Mushroom Research Centre, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Murali Naidu
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Vikineswary Sabaratnam
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Sharmili Vidyadaran
Mushroom Research Centre, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Selangor, Malaysia
Kah-Hui Wong
Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Mushroom Research Centre, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

要約

In Malaysia and China, the sclerotium of Lignosus rhinocerotis is used by local communities and traditional medicine practitioners as a general tonic and remedy to treat a variety of ailments, including inflammation-associated disorders. In this study, 10 samples from different preparations of L. rhinocerotis sclerotium, including a hot aqueous extract (HAE), an ethanol extract (EE), fractions from the HAE and EE, and crude polysaccharides, were tested for their in vitro cytotoxic and nitric oxide (NO) inhibitory activities in lipopolysaccharide (LPS)−stimulated BV2 microglia. Of the 10 samples tested, HAE was the least cytotoxic toward BV2 microglia, with a half-maximal inhibitory concentration of 176.23 ± 2.64 mg/mL at 24 hours of incubation and 20.01 ± 1.69 mg/ mL at 48 hours of incubation. The inhibition of NO production was explored by pretreatment of BV2 microglia with samples at 2 incubation time points (4 and 24 hours) before the stimulation by LPS for 24 hours. After 24 hours of pretreatment, 8 of the 10 samples inhibited NO production by 50% or more, and cytotoxic effects were not observed. Among the 8 active samples, 500 µg/mL of HAE, 250 µg/mL of an n-butanol fraction of the HAE, and 250 µg/mL of an ethyl acetate fraction of HAE showed maximum inhibition of NO production by 88.95%, 86.50%, and 85.93%, respectively. These results suggest that the L. rhinocerotis sclerotium may contain secondary metabolites that have the potential to inhibit NO production.


Articles with similar content:

Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 6
Tatjana P. Stanojkovic, Tatjana D. Kundakovic, Milan Kojic, Marina D. Kolundzic, Marina T. Milenkovic, Nada O. Grozdanic, Miroslav R. Dinic, Natasa E. Golic
Medicinal Mushroom Extracts Possess Differential Antioxidant Activity and Cytotoxicity to Cancer Cells
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Eglal AbdAllah Ghonimy, Mahomud Mohammed Alassar, Fang-Sheng Wu, Eman Nasr Elbatrawy
In Vitro Anti-Helicobacter pylori Effects of Medicinal Mushroom Extracts, with Special Emphasis on the Lion's Mane Mushroom, Hericium erinaceus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Qi Tan, Kangying Yu, Pingzuo Li, Ruina Liu, Xiaodong Shang, Guo-Ping Zhao
Lion's Mane, Hericium erinaceus and Tiger Milk, Lignosus rhinocerotis (Higher Basidiomycetes) Medicinal Mushrooms Stimulate Neurite Outgrowth in Dissociated Cells of Brain, Spinal Cord, and Retina: An In Vitro Study
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 11
Snehlata Samberkar, Kah-Hui Wong, Sivasangkary Gandhi, Jegadeesh Raman, Murali Naidu, Vikineswary Sabaratnam
Antioxidant and Immunomodulating Activities of Exo-and Endopolysaccharide Fractions from Submerged Mycelia Cultures of Culinary-Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 3
Karthik Marripudi, Cheang Khoo, Sundar Rao Koyyalamudi, Jong Pil Park, Jin Hee Kim, Sang-Chul Jeong, Chi-Hyun Song, J. Margaret Hughes, Trevor Bailey