ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i5.70
pages 457-465

A Validated Reverse-Phase HPLC Method for Quantitative Determination of Ganoderic Acids A and B in Cultivated Strains of Ganoderma spp. (Agaricomycetes) Indigenous to India

M. Ramakrishna
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
D. Rajesh Babu
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
S. S. Veena
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Meera Pandey
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Nageswara Rao
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India

要約

Twenty isolates of Ganoderma spp. indigenous to India were used in this study. A reverse-phase high-performance liquid chromatography (HPLC) method was used to quantify 2 of the most bioactive triterpenes, ganoderic acid A (GAA) and ganoderic acid B (GAB), among the cultivated Ganoderma spp. The HPLC analysis was performed on an Agilent 1260 Infinity HPLC system with a Zorbax C18 column, using gradient elution of acetonitrile and 0.1% acetic acid. The detection wavelength was set at 254 nm, with a flow rate of 0.6 mL/min. The method was validated according to the guidelines of International Conference on Harmonisation and produced satisfactory results. The amount of GAA varied from 827.50 to 2010.36 µg/g, whereas GAB varied from 16.64 to 916.89 µg/g. The developed method is simple, specific, accurate, and precise, and can be useful for qualitative and quantitative evaluation of ganoderic acids.


Articles with similar content:

Chemical Analysis as a Quality Control Method for Medicinal Mushroom and Fungi Extracts
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 3
John Seleen, Xian-guo He
Study on the Analytical Method for Water-soluble Crude Polysaccharides in Culinary-Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 4
Shu-Chi Tsai, Weiguang Fu, Shu-Fang Lu, Hsiao-Chen Lin
Larvicidal, Ovicidal, and Histopathological Effects of the Sulphur Polypore Mushroom, Laetiporus sulphureus (Agaricomycetes), Collected from Tamil Nadu, India against Mosquitoes
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 12
Savarimuthu Ignacimuthu, Pathalam Ganesan, Michael Gabriel Paulraj, Subramaniyan Sivanandhan
Screening for Antimicrobial Activity of Wood Rotting Higher Basidiomycetes Mushrooms from Uruguay against Phytopathogens
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 3
Alvaro Vázquez, María Pía Cerdeiras, Gianna Cecchetto, Stephanie Barneche, Gabriela Jorcin, Silvana Alborés
Cytotoxic Activities of Ergosta-4, 6, 8 (14), 22-tetraen-3-one from the Sclerotia of Grifola umbellata (Pers.) Pilát
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Kang Hyeon Ka, Youngki Park, Jin Kwon Ahn, Wi Young Lee, Hak Ju Lee