ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i8.80
pages 783-797

Bioinformatic Identification of Potential MicroRNAs and Their Targets in the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes)

Dashuai Mu
College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing, Jiangsu, People's Republic of China; College of Marine Science, Shandong University (Weihai), Weihai, People's Republic of China
Chenyang Li
College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing, Jiangsu, People's Republic of China
Liang Shi
College of Life Sciences, Nanjing Agricultural University; Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, Nanjing 210095, Jiangsu, People's Republic of China
Xuchen Zhang
College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing, Jiangsu, People's Republic of China
Ang Ren
Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing, Jiangsu, People's Republic of China
Mingwen Zhao
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China

要約

MicroRNAs (miRNAs) are a class of small, endogenous, noncoding RNA molecules that negatively regulate gene expression at the transcriptional or the post-transcriptional level. Although a large number of miRNAs have been identified in many species, especially model plants and animals, miRNAs in fungi remain largely unknown. In this study, based on a database of expressed sequence tags in Ganoderma lucidum, 89 potential miRNAs were identified using computational methods. Real-time polymerase chain reaction analysis of miRNA-like samples prepared from G. lucidum at different development stages revealed that miRNA-like RNAs were differentially expressed in different stages. Furthermore, a total of 28 potential targets were found based on near-perfect or perfect complementarity between the randomly selected 9 miRNA-like RNAs and the target sequences, and potential targets for G. lucidum miRNA-like RNAs were predicted. Finally, we studied the expression pattern of 4 target genes in 3 different development stages of G. lucidum to further understand the mechanism of interaction between miRNA-like RNAs and their target genes. Our analysis paves the way toward identifying fungal miRNA-like RNAs that might be involved in various physiological and cellular differentiation processes.


Articles with similar content:

Gene Prioritization and Network Topology Analysis of Targeted Genes for Acquired Taxane Resistance by Meta-Analysis
Critical Reviews™ in Eukaryotic Gene Expression, Vol.29, 2019, issue 6
Jin Ki Kim, Young Seok Lee, Dongha Kim, Sung Young Kim
Multiple-Scales Integrative Analysis of MicroRNAs Unveils Biomarkers and Key Regulatory Connections for Hepatocellular Carcinoma
Critical Reviews™ in Eukaryotic Gene Expression, Vol.29, 2019, issue 3
Xiaohong Xiang, Wanyan Deng, Keli Qian, Qian Li
Common Variants rs3815188 and rs1043994 on Notch3 Gene Confer Susceptibility to Lung Cancer: A Hospital-Based Case–Control Study
Journal of Environmental Pathology, Toxicology and Oncology, Vol.38, 2019, issue 1
Faruk Saydam, Guntulu Ak, Cansu Ozbayer, Irfan Degirmenci, Muzaffer Metintas, Emine Yagci
Identification of Reference Genes and Analysis of Heat Shock Protein Gene Expression in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, after Exposure to Heat Stress
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 11
Mingwen Zhao, Ai-Liang Jiang, Xiao-Xiao Lu, Yong-Nan Liu, Liang Shi, Ang Ren, Han-Shou Yu
The Impacts of miRNAs in Glioblastoma Progression
Critical Reviews™ in Eukaryotic Gene Expression, Vol.26, 2016, issue 2
Rasime Kalkan, Emine İkbal Atli