ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i8.50
pages 749-761

Submerged Cultivation of Mycelium with High Ergothioneine Content from the Culinary-Medicinal Golden Oyster Mushroom, Pleurotus citrinopileatus (Higher Basidiomycetes)

Shin-Yi Lin
Department of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, Republic of China; NCHU-UCD Plant and Food Biotechnology Program, Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, Republic of China; Agricultural Biotechnology Center, NCHU, Taiwan, R.O.C
Shih-Chang Chien
The Experimental Forest Management Office, NCHU, Taichung, Taiwan, R.O.C
Sheng-Yang Wang
Department of Forestry, National Chung Hsing University, Taichung, Taiwan, Republic of China
Jeng-Leun Mau
Department of Food Science and Biotechnology National Chung-Hsing University 250 Kuokuang Road, Taichung 40227, Taiwan, ROC

要約

The optimization of submerged culture of the culinary-medicinal golden oyster mushroom, Pleurotus citrinopileatus, was studied using a one-factor-at-a-time, two-stage stimulation and central composite rotatable design to produce mycelia with high ergothioneine content. The optimal culture conditions for mycelia harvested at day 22 were a temperature of 25°C, an inoculation ratio of 5%, 2% glucose, 0.5% yeast extract, and adjustment of the initial pH value to 10. The biomass and ergothioneine content were 8.28 g/L and 10.65 mg/g dry weight (dw), respectively. The addition of an amino acid precursor increased the ergothioneine content of mycelia; cysteine was the most effective. In addition, the results obtained from central composite rotatable design showed that the recommended combination for cysteine, histidine, and methionine was 8, 4, and 0.5 mmol/L, respectively. The predicted ergothioneine content was 13.90 mg/g dw, whereas the experimental maximal ergothioneine content was 14.57 mg/g dw. With the addition of complex precursors and under optimal culture conditions, mycelia harvested at days 16−20 had higher ergothioneine content. Accordingly, the information obtained could be used to produce mycelia with high ergothioneine content.


Articles with similar content:

Submerged Cultivation of Mycelium with High Ergothioneine Content from the Culinary-Medicinal King Oyster Mushroom Pleurotus eryngii (Higher Basidiomycetes) and its Composition
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Shin-Yi Lin, Jeng-Leun Mau, Ling-Yi Huang, Kung-Jui Ho, Chih-Hung Liang
The Influence of Cultivation Conditions on Mycelial Growth and Exopolysaccharide Production of Culinary-Medicinal Mushroom Pleurotus citrinopileatus Singer (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 3
Chiu-Yeh Wu
Chemical Composition and Medicinal Value of Fruiting Bodies and Submerged Cultured Mycelia of Caterpillar Medicinal Fungus Cordyceps militaris CBS-132098 (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 7
Solomon P. Wasser, Mikheil D. Asatiani, Gayane S. Barseghyan, Jannie Siew Lee Chan
Productivity and Nutritional Content of Culinary-Medicinal Oyster Mushroom Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae) Fruit Bodies Cultivated on Substrates Containing Solid Waste from Anaerobic Digested Poultry Litter
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 2
Nona A. Mikiashvili, Omoanghe S. Isikhuemhen
Chemical Composition and Nutritional and Medicinal Value of Fruit Bodies and Submerged Cultured Mycelia of Culinary-Medicinal Higher Basidiomycetes Mushrooms
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 3
Nachshol Cohen, Jacob Cohen, Yu-Hsuan Li, Solomon P. Wasser, Jeng-Leun Mau, Yi-Chi Yang, Hui-Tzu Yu, Mikheil D. Asatiani, Vinay K. Varshney