ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i2.60
pages 157-165

Composition Analysis and Immunomodulatory Capacity of Peptidoglycan from Ling Zhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. Strain 119 (Aphyllophoromycetideae)

Libin Ye
University of Illinois at Urbana-Champaign; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi; and Key Laboratory of Edible Fungi Resources and Utilization (South), People's Republic of China Ministry of Agriculture, Shanghai, China; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China
Xiaolin Zheng
College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou 310035, China
Jing-Song Zhang
National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Yan Yang
National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Yuecheng Meng
College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou 310035, China
Jianrong Li
College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou 310035, China
Wei Chen
College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou 310035, China
Ang Li
College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou 310035, China
Ying-jie Pan
College of Food Sciences, Shanghai Ocean University, Shanghai 200090, China

要約

LZ-B-1, a water-soluble peptidoglycan, was prepared from the fruiting bodies of Ling Zhi or Reishi medicinal mushroom, Ganoderma lucidum, by ultrafiltration, DEAE-Sepharose Fast-Flow column, and Sepharose CL-6B column. To develop functional food or pharmaceuticals using LZ-B-1 and to investigate the relationship between the structure and bioactivity of LZ-B-1, the composition and immunomodulatory capacity of LZ-B-1 were studied herein. The peptidoglycan had a relative molecular weight of 1.12 × 104 Da, and total carbohydrate and peptide percentages were determined to be ∼94.2% and ∼5.4%, respectively. The carbohydrate moiety was mainly composed of L-fucose, D-galatose, and D-glucose in a molar ratio of approximately 1:5:2, together with small amounts of L-arabinose and D-mannose. The peptide moiety mainly contained arginine, threonine, glycine, serine, and aspartic acid in a molar ratio of approximately 1:1:2:2:1. Methylation reaction and GC-MS analysis indicated that the carbohydrate moiety mainly consisted of 1,6-disubstituted-galactopyranosyl, 1,2,6-trisubstituted-galactopyranosyl, 1-substituted-fucopyranosyl, 1,3-disubstituted- glucopyranosyl, 1,4,6-trisubstituted-glucopyranosyl, and 1-substituted-glucopyranosyl. Mouse spleen cell proliferation assay in vitro showed that a concentration of 200 μg/mL of LZ-B-1 was the optimum concentration to promote the proliferation of mouse spleen lymphocytes (MSLs). In general, the higher the mouse spleen cell proliferation rate, the stronger the immunomodulatory activity. How the peptidoglycan stimulates the proliferation of MSLs and the bioactivity-structure relationship requires more research.


Articles with similar content:

Chemical Composition and Immunomodulatory Activity of Mycelia of the Hairy Bracket Mushroom, Trametes hirsuta (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 3
Rongxia Ma, Zhiyi Chen, Rongling Yang, Siyuan Wang, Chunying Yang, Xueming Liu
Polysaccharides and Antioxidants from Culinary-Medicinal White Button Mushroom, Agaricus bisporus (Agaricomycetes), Waste Biomass
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 8
Jasenka Piljac-Zegarac, Vlatka Petravić-Tominac, Senka Djakovic, Vesna Zechner-Krpan, Sinisa Srecec, Aleksandra Vojvodic Cebin, Omoanghe S. Isikhuemhen
High-Level Production of Exopolysaccharides by a Cosmic Radiation−Induced Mutant M270 of the Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 7
Ai-Rong Song, Chen Zhao, Wen-Xing Liang, Guang-Yuan Wang, Xue-Mei Tian
Chemical Characterization and Structure of Exopolysaccharides from Submerged Culture of New Medicinal Mushroom from China, Phellinus mori (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 1
Feng Peng, Bao-Kai Cui, Chunlei Cao
Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 4
Jie Qiao, Dayong Xu, Huawei Zeng, Yuying Shuai, Feng Li, Xin Zeng, Shengqi Rao