ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i2.130
pages 213-222

Comparative Cytotoxicity and Hemagglutination Activities of Crude Protein Extracts from Culinary-Medicinal Mushrooms

Suan Li Lee
Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, Cheras, 56000 Kuala Lumpur, Malaysia
Jin Ying Leong
Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, Cheras, 56000 Kuala Lumpur, Malaysia
Renee Lay Hong Lim
Faculty of Applied Sciences, UCSI University, Malaysia

要約

The bioactivities of crude protein extracts from three commercial culinary-medicinal mushrooms, Flammulina velutipes, Pleurotus citrinopileatus, and Hericium erinaceus, were compared in this study to determine the presence of new and effective anticancer and immunomodulatory agents in these mushroom species. F. velutipes was used as a reference mushroom for its fungal immunomodulatory protein (FIP-fve), reported to exhibit potent mitogenic, cytotoxicity, and hemagglutination activities. The total yield of crude protein extracts obtained from F. velutipes, P. citrinopileatus, and H. erinaceus were 0.255, 0.501, and 0.369 mg/g of fresh fruiting bodies, respectively. SDS-PAGE profile analysis for protein bands having molecular weight in the range reported for the FIPs family revealed that F. velutipes and P. citrinopileatus crude protein extracts contained a distinct 13-kDa FIP-fve and a 15-kDa FIP from P. citrinopileatus (PCiP), respectively. However, faint 10- to 14-kDa protein bands were observed in crude extract from H. erinaceus. Crude protein extracts of F. velutipes, P. citrinopileatus, and H. erinaceus showed cytotoxicity on human cervical cancer HeLa cells at IC50 concentrations of 10.0, 21.6, and 31.2 μg/mL, respectively. The trypan blue exclusion assay further confirmed that protein extracts from F. velutipes and P. citrinopileatus, compared to H. erinaceus, showed significantly higher cytotoxicity on HeLa cells. Of the three species studied, only F. velutipes crude extract caused hemagglutination of human whole blood cells, possibly due to the FIP-fve. As there are no previous reports of cytotoxicity of these mushrooms on HeLa cells, our findings provided new insights into the presence of potential anticancer components in these mushrooms, which can be subsequently purified for further analysis and identification.


Articles with similar content:

Anti-Inflammatory and Antimicrobial Activities of Aqueous Extracts of Wild Mushrooms from Japan
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 5
Zhihao Tu, Dagula Hu, Chiaki Takeuchi, Sayumi Yamada, Toshihiro Hoshiba, Rina Miura, Akihiro Yamaguchi, Keita Matsuoka, Ryota Sugawara, Mai Tanaka
Anti-inflammatory Activity of Mycelial Extracts from Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 4
Hongyu Xu, Zhenming Lu, Yan Geng, Zheng-Hong Xu, Jin-Song Shi, Shuiling Zhu
Quantification of Water-Soluble Metabolites in Medicinal Mushrooms Using Proton NMR Spectroscopy
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 5
Carolyn M. Slupsky, Yu-Chang Lo, Jeng-Leun Mau, Shih-Chang Chien, Darya O. Mishchuk
Antioxidant, Anti-Inflammatory, and Antitumor Activities of Cultured Mycelia and Fruiting Bodies of the Elm Oyster Mushroom, Hypsizygus ulmarius (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 3
Kainoor K. Janardhanan, Korattuvalappil S. Ravikumar, Karattuthodi Fathimathu Zuhara, Panavalappil Greeshma, Mangalathmelathil N. Neethu, Meera Pandey
Antioxidant and Cytotoxic Activities of Ethanolic Extracts and Isolated Fractions of Species of the Genus Phellinus Quél. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 2
Yanfang Liu, Yan Yang, Libin Ye, Jing-Song Zhang, Jinxia Hu, Na Feng, Hui Chen, Qing-jiu Tang Tang