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International Journal of Medicinal Mushrooms

年間 12 号発行

ISSN 印刷: 1521-9437

ISSN オンライン: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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A Lectin Purified from Blood Red Bracket Mushroom, Pycnoporus sanguineus (Agaricomycetidae), Mycelium Displayed Affinity Toward Bovine Transferrin

巻 18, 発行 1, 2016, pp. 67-74
DOI: 10.1615/IntJMedMushrooms.v18.i1.80
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要約

Fungal lectins constitute excellent ligands for development of affinity adsorbents useful in affinity chromatography. In this work, a lectin was purified from Pycnoporus sanguineus (PSL) mycelium using 3 procedures: by affinity chromatography, using magnetic galactosyl-nanoparticles or galactose coupled to Sepharose, and by ionic exchange chromatography (IEC). The highest lectin yield was achieved by IEC (55%); SDS-PAGE of PSL showed 2 bands with molecular mass of 68.7 and 55.2 kDa and IEC displayed 2 bands at pi 5.5 and 5.2. The lectin agglutinates rat erythrocytes, exhibiting broad specificity toward several monosaccharides, including galactose. The agglutination was also inhibited by the glycoproteins fetal calf fetuin, bovine lactoferrin, bovine transferrin, and horseradish peroxidase. The lectin was then used to synthesize an affinity adsorbent (PSL-Sepharose) and the interaction with glycoproteins was evaluated by analyzing their chromatographic behaviors. The strongest interaction with the PSL-derivative was observed with transferrin, although lower interactions were also displayed toward fetuin and lactoferrin. These results indicate that the purified PSL constitutes an interesting ligand for the design of affinity adsorbents to be used (i.e., in glycoprotein purification).

によって引用された
  1. Nikitina Valentina, Loshchinina Ekaterina, Vetchinkina Elena, Lectins from Mycelia of Basidiomycetes, International Journal of Molecular Sciences, 18, 7, 2017. Crossref

  2. Phan Chia-Wei, Wang Joon-Keong, Tan Elson Yi-Yong, Tan Yee-Shin, Sathiya Seelan Jaya Seelan, Cheah Shiau-Chuen, Vikineswary Sabaratnam, Giant oyster mushroom,Pleurotus giganteus(Agaricomycetes): Current status of the cultivation methods, chemical composition, biological, and health-promoting properties, Food Reviews International, 35, 4, 2019. Crossref

  3. Singh Ram Sarup, Walia Amandeep Kaur, Kennedy John F., Mushroom lectins in biomedical research and development, International Journal of Biological Macromolecules, 151, 2020. Crossref

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