年間 12 号発行
ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344
Indexed in
A Lectin Purified from Blood Red Bracket Mushroom, Pycnoporus sanguineus (Agaricomycetidae), Mycelium Displayed Affinity Toward Bovine Transferrin
要約
Fungal lectins constitute excellent ligands for development of affinity adsorbents useful in affinity chromatography. In this work, a lectin was purified from Pycnoporus sanguineus (PSL) mycelium using 3 procedures: by affinity chromatography, using magnetic galactosyl-nanoparticles or galactose coupled to Sepharose, and by ionic exchange chromatography (IEC). The highest lectin yield was achieved by IEC (55%); SDS-PAGE of PSL showed 2 bands with molecular mass of 68.7 and 55.2 kDa and IEC displayed 2 bands at pi 5.5 and 5.2. The lectin agglutinates rat erythrocytes, exhibiting broad specificity toward several monosaccharides, including galactose. The agglutination was also inhibited by the glycoproteins fetal calf fetuin, bovine lactoferrin, bovine transferrin, and horseradish peroxidase. The lectin was then used to synthesize an affinity adsorbent (PSL-Sepharose) and the interaction with glycoproteins was evaluated by analyzing their chromatographic behaviors. The strongest interaction with the PSL-derivative was observed with transferrin, although lower interactions were also displayed toward fetuin and lactoferrin. These results indicate that the purified PSL constitutes an interesting ligand for the design of affinity adsorbents to be used (i.e., in glycoprotein purification).
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Nikitina Valentina, Loshchinina Ekaterina, Vetchinkina Elena, Lectins from Mycelia of Basidiomycetes, International Journal of Molecular Sciences, 18, 7, 2017. Crossref
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Phan Chia-Wei, Wang Joon-Keong, Tan Elson Yi-Yong, Tan Yee-Shin, Sathiya Seelan Jaya Seelan, Cheah Shiau-Chuen, Vikineswary Sabaratnam, Giant oyster mushroom,Pleurotus giganteus(Agaricomycetes): Current status of the cultivation methods, chemical composition, biological, and health-promoting properties, Food Reviews International, 35, 4, 2019. Crossref
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Singh Ram Sarup, Walia Amandeep Kaur, Kennedy John F., Mushroom lectins in biomedical research and development, International Journal of Biological Macromolecules, 151, 2020. Crossref