ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i3.70
pages 279-286

Anti-Human Papillomavirus (HPV) 16 E6 Activity of Ling Zhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Extracts

Li Kuan Lai
Institute of Biological Sciences, Faculty of Science, University Malaya, Kuala Lumpur, Malaysia
Nurhayati Zainal Abidin
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Noorlidah Abdullah
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Vikineswary Sabaratnam
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

要約

Cancer of the cervix ranks second in the demographics of cancers affecting women worldwide. Human papillomavirus (HPV) type 16 is one of the major causative agents for cervical cancer. The E6 region of the HPV 16 genome encodes for an oncoprotein responsible for the pathogenesis of the disease. The E6 oncoprotein is abundant in cervical cancer and cervical cancer-derived cell lines. Mushrooms of the genus Ganoderma have long been used traditionally as an immunomodulating and antitumor agent, with recent studies validating its use in chemotherapeutics. Due to the importance of the E6 oncoprotein in the carcinogenesis of cervical cancer, this study was undertaken to screen the crude dichloromethane, ethanol, water, and polysaccharide extracts of Ganoderma lucidum for the ability to suppress the expression of HPV 16 E6 and to evaluate secondary metabolite classes present in the active crude extract. CaSki cells were treated with the crude extracts and were then subjected to immunocytochemistry protocols. All crude extracts of G. lucidum presented HPV 16 E6 suppression, with the dichloromethane extract being the most active compared to the other crude extracts. The crude dichloromethane extract was then subjected to thin-layer chromatography to evaluate the secondary metabolite classes present. The present study shows that the crude dichloromethane extract of G. lucidum possesses flavonoids, terpenoids, phenolics, and alkaloids. Further isolation work on the dichloromethane extract of G. lucidum could direct us to lead compounds with promising anti-HPV 16 E6 activity.


Articles with similar content:

Inhibitory Activity of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) on Transformed Cells by Human Papillomavirus
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 2
Vicente Madrid-Marina V., Elizur Montiel-Arcos, Ismael Leon-Rivera, Raul N. Ondarza-Vidaurreta, Enrique Garcia-Villa, Victor H. Bermudez-Morales, Eva Hernandez-Marquez, Patricio Gariglio, Ana I. Burgete-Garcfa, Alfredo Lagunas-Martinez
Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), as a Cardioprotectant in an Oxygen-Deficient Environment
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 11
Kshipra Misra, Sarita Nehra, Jigni Mishra, Vandana Kirar, Rakhee, Deepika Saraswat
Comparative Cytotoxicity and Hemagglutination Activities of Crude Protein Extracts from Culinary-Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 2
Renee Lay Hong Lim, Suan Li Lee, Jin Ying Leong
Cytotoxic Activities of Fractions of the Willow Bracket Medicinal Mushroom, Phellinus igniarius (Agaricomycetes), and the Induction of Cell Cycle Arrest and Apoptosis in MGC-803 Cells
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 6
Fei-Fei Wang, Wei Ma, Ning Li , Feng Liu, Chao Shi, Kai-Jin Wang
Antiproliferative Activity of Some Higher Mushrooms from Hungary against Human Cancer Cell Lines
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Attila Vanyolos, Istvan Zupko, Noemi Bozsity, Judit Hohmann, Bernadett Kovacs