年間 12 号発行
ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344
Indexed in
Development of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) Polysaccharides Injection Formulation
要約
Biochemical and pharmacological research has demonstrated that Lingzhi or Reishi medicinal mushroom Ganoderma lucidum polysaccharides (GLPS) have significant anticancer, antitumor, and antioxidant activities. To investigate the effect of injecting GLPS into hosts for clinical studies, aqueous polysaccharide extracts from G. lucidum fruit bodies were purified by deproteinization using the Sevage method, anion-exchange chromatography elution (cellulose DEAE-52 chromatography), dialysis, ethanol precipitation, and active carbon and millipore membrane filtration techniques. The purified GLPS were used for injection in mice. Polysaccharide indexes, protein, tannin, heavy metal, arsenic salt, oxalate, potassium ion, resin, pH, ignition residue measurements, evaluation criterion for allergic reactions, and total solids content of the GLPS injection were all performed using the reference methods in the Chinese Pharmacopoeia. Our results showed that polysaccharide was the key component of injection mixtures. The ignition residue and total solids content in the injection mixture were 1.4% and 2.4%, respectively. The other indices were all within the expected safety ranges. Furthermore, studies from mice functional assays showed that the injection mixture improved the antifatigue capacity of mice without any effect on weight loss/gain. In addition, the injection mixture was safe, which was confirmed by allergy testing in guinea pigs. The development of a GLPS injection offers a novel approach for future medicinal mushroom utilization and holds great commercial promise.
-
Morris Humberto J., Llauradó Gabriel, Beltrán Yaixa, Lebeque Yamila, Bermúdez Rosa C., García Nora, Gaime-Perraud Isabelle, Moukha Serge, The Use of Mushrooms in the Development of Functional Foods, Drugs, and Nutraceuticals, in Wild Plants, Mushrooms and Nuts, 2016. Crossref
-
Friedman Mendel, Mushroom Polysaccharides: Chemistry and Antiobesity, Antidiabetes, Anticancer, and Antibiotic Properties in Cells, Rodents, and Humans, Foods, 5, 4, 2016. Crossref
-
Luo Chuanhong, Xu Xiaorong, Wei Xichuan, Feng Wuwen, Huang Haozhou, Liu Haiyan, Xu Runchun, Lin Junzhi, Han Li, Zhang Dingkun, Natural medicines for the treatment of fatigue: Bioactive components, pharmacology, and mechanisms, Pharmacological Research, 148, 2019. Crossref
-
Yang Ke, Zhan Lianghui, Lu Tingting, Zhou Cong, Chen Xue, Dong Yingjie, Lv Guiyuan, Chen Suhong, Dendrobium officinale polysaccharides protected against ethanol-induced acute liver injury in vivo and in vitro via the TLR4/NF-κB signaling pathway, Cytokine, 130, 2020. Crossref
-
Huang Jiafu, Ou Yixin, Yew Tai Wai David, Liu Jingna, Leng Bo, Lin Zhichao, Su Yi, Zhuang Yuanhong, Lin Jiaofen, Li Xiumin, Xue Yu, Pan Yutian, Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl4-induced hepatic injury in mice, International Journal of Biological Macromolecules, 82, 2016. Crossref
-
Zhao Hengqiang, Lai Chang-Jiang-Sheng, Yu Yi, Wang Ya-nan, Zhao Yu-Jun, Ma Fangli, Hu Minghua, Guo Juan, Wang Xiao, Guo Lanping, Acidic hydrolysate fingerprints based on HILIC-ELSD/MS combined with multivariate analysis for investigating the quality of Ganoderma lucidum polysaccharides, International Journal of Biological Macromolecules, 163, 2020. Crossref
-
Ahmad Rizwan, Riaz Muhammad, Khan Aslam, Aljamea Ahmed, Algheryafi Mohammad, Sewaket Deya, Alqathama Aljawharah, Ganoderma lucidum (Reishi) an edible mushroom; a comprehensive and critical review of its nutritional, cosmeceutical, mycochemical, pharmacological, clinical, and toxicological properties , Phytotherapy Research, 35, 11, 2021. Crossref