ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

巻:
巻 22, 2020 巻 21, 2019 巻 20, 2018 巻 19, 2017 巻 18, 2016 巻 17, 2015 巻 16, 2014 巻 15, 2013 巻 14, 2012 巻 13, 2011 巻 12, 2010 巻 11, 2009 巻 10, 2008 巻 9, 2007 巻 8, 2006 巻 7, 2005 巻 6, 2004 巻 5, 2003 巻 4, 2002 巻 3, 2001 巻 2, 2000 巻 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v16.i3.90
pages 293-302

The Optimum Submerged Culture Condition of the Culinary-Medicinal White Jelly Mushroom (Tremellomycetes) and Its Antioxidant Properties

Jessica Krisanti Gusman
Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan
Chien-Yih Lin
Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan
Yang-Chia Shih
Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan

要約

Tremella fuciformis is a natural edible and medicinal mushroom commercially available in Taiwan. In this study, the effects of initial pH, incubation time, and various media to optimize exopolysaccharide (EPS) production by T. fuciformis were evaluated in shake flasks and a bioreactor. The antioxidant properties were investigated. The results showed that using potato dextrose broth medium (pH 9) and a 48-hour incubation time in a shake flask was the most efficient condition from which to obtain maximum EPS. However, the quantity of EPS from different initial pH values (5−9) was not significantly different. Total polyphenol and β−glucan contents from every EPS sample (pH 5−9) were evaluated. T. fuciformis polysaccharides (10 mg/mL, pH 9) could scavenge 40.63% of 1,1-diphenyl-2-picrylhydrazyl radicals. The chelating abilities of ferrous ion of all extracts reached more than 80%. EPS extract from the initial pH value of 9 showed the highest reducing power among the other pH values (half maximal effective concentration = 5.97 mg/mL). The EPS from T. fuciformis was noncytotoxic to mouse skin fibroblasts (NIH/3T3), and survival rates were more than 100% using MTT assay. The samples were used to analyze the scavenging activity against oxidative damage induced by ultraviolet B radiation and hydrogen peroxide. The results showed the antioxidant activity occurred in a dose-dependent manner (P < 0.05). Furthermore, these findings prove that the EPS of T. fuciformis from submerged culture possess antioxidant properties and can be used as an alternative treatment for cell protection.


Articles with similar content:

Preparation of Culinary-Medicinal Maitake Mushroom, Grifola frondosa (Dicks.: Fr.) S.F. Gray (Aphyllophoromycetideae)-fermented Wheat and its Antioxidant Properties
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 1
Shin-Yi Lin, Pei-Ying Lian, Jeng-Leun Mau, Shu-Yao Tsai, Chih-Hung Liang, Shih-Jeng Huang
Antioxidant and Antiradical Properties of Methanolic Extracts from Algerian Wild Edible Desert Truffles (Terfezia and Tirmania, Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 5
Hicham Gouzi, Mourad Leboukh, Elmouloud Bouchouka
Optimization of the Production of Extracellular Polysaccharide from the Shiitake Medicinal Mushroom Lentinus edodes (Agaricomycetes) Using Mutation and a Genetic Algorithm−Coupled Artificial Neural Network (GA-ANN)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 7
Agbaje Lateef, Adeyemi Ojutalayo Adeeyo, Evariste Bosco Gueguim-Kana
Antidiabetic and Antioxidant Activities of Eight Medicinal Mushroom Species from China
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 2
Baojun (Bruce) Xu, Tong Wu
Comparison of the Chemical Profiles and Antioxidant and Antidiabetic Activities of Extracts from Two Ganoderma Species (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 7
Baojun (Bruce) Xu, Xiaoqing Tang, Weixi Cai