ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v16.i3.40
pages 239-245

In Vitro Anti-proliferative Effects on NB4 Human Leukemia Cells and Physicochemical Screening of Pleurotus sp. (Higher Basidiomycetes) Mycelia from Cuba

Humberto J. Morris
Centre of Studies for Industrial Biotechnology, Universidad de Oriente, Santiago de Cuba, Cuba
Edgar Hernandez
Department of Biology, Faculty of Natural Sciences, Universidad de Oriente, Santiago de Cuba 5. CP 90 500, Cuba
Gabriel Llaurado
Centre of Studies for Industrial Biotechnology, Universidad de Oriente, Santiago de Cuba, Cuba
Maria Cristina Tejedor
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Pilar Sancho
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Angel Herraez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Maria del Carmen Boyano-Adanez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Ana Isabel Garcia-Perez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Jose Carlos Diez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain

要約

This study examined the phytochemical profile and the in vitro anti-proliferative effects of a hot water mycelial extract from the edible mushroom Pleurotus sp. on NB4 human leukemia cells. Flow-cytometry analyses were used to measure cell viability, cell cycle, and apoptosis in cells incubated 24 h with the extract at doses of 100 and 200 µg/mL. Pleurotus sp. extract reduced cell viability, particularly at the concentration of 200 µg/mL to 82% compared to control cells, and induced apoptosis demonstrated by an increase in the number of annexin V-FITC+ cells (25% at 200 µg/mL). The NB4 cells were arrested in the G2/M phase thus supporting a cell-cycle dependent anticancer mechanism. Although carbohydrates (76.8%, w/w) appear to be the most important antitumor compound, secondary metabolites-like phenolics would also contribute to the anti-proliferative activity. The results indicate that Pleurotus sp. mycelia obtained by submerged fermentation may be an interesting renewable resource for developing functional foods and new antitumor therapeutic agents.


Articles with similar content:

The Effect of Culture Liquid Ethyl Acetate Mycelium Extracts of Medicinal Mushrooms on the Viability of Human Pancreatic Cancer Cells
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 2
Solomon P. Wasser, Lital E. Sharvit, Fuad Fares
Autophagy Inhibition Enhances SPCA-1 Cell Proliferation Inhibition Induced by By-1 from the Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 4
Di Wu, Wenbo Sun, Yanfang Liu, Yaping Zhang, Yan Yang, Henan Zhang, Li Min, Hairui Yang, Zheng Qiankun, Wei Jia, Wenhan Wang, Jinsong Zhang
Bioactive Components of Ganoderma lucidum (W.Curt.:Fr.) Lloyd Can Induce Apoptosis of Tumor Cells
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Martin Zimmerman-Kordmann, Jing-Song Zhang, Hua Fan, Werner Reutter, Qing-jiu Tang Tang
Comparative Study on the Effect of Aqueous and Ethanolic Mycelial Extracts from Polycephalomyces nipponicus (Ascomycetes) against Human Breast Cancer MCF-7 Cells
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 7
Benjaporn Buranrat, Aphidech Sangdee, Kusavadee Sangdee
A Comparison Study of the Anticancerous Activity and Mechanism of Ethanolic Extracts from Different Ganoderma lucidum (W.Curt.:Fr.) Lloyd Strains
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Yang Qing-Yao, Mi Ke, Feng Hui-Qing, Zhou Yue-Qing, Xiao-Tong Yang, Xu-Quan Li