年間 4 号発行
ISSN 印刷: 2155-014X
ISSN オンライン: 2155-0158
The Peculiarities of Arginase Pathway of L-Arginine Metabolism in Spermatozoa of Men with Different Forms of Pathospermia
要約
The changes in arginase activity of spermatozoa and hormonal profile of peripheral blood of infertile men with various forms of pathospermia were studied. It has been found that arginase activity in the sperm cells of men with oligozoo-, antenozoo-, oligoastenozoo- and leucocytospermia decreases 2.1, 2.3, 2.4, and 3.3 times, respectively, indicating the inhibition of arginase pathway of L-arginine metabolism, which is not significantly dependent on the type of spermatogenesis disruption. The most significant changes have been observed in infertile men with leucocytospermia, since leukocytes stimulate the formation of reactive oxygen species, induction and development of oxidative and nitrosative stress in spermatozoa. Inhibiting of arginase pathway of L-arginine metabolism plays an adaptive role, which is to limit bioavailability of L-arginine and prevent excessive formation of NO in cytotoxic to sperm cells concentrations. The changes in serum concentrations of gonadotropin and sex hormones in men with various forms of pathospermia have been also noted. In particular, the content of follicle stimulating hormone and testosterone in patients with oligozoospermia induced by hypogonadism is twice higher, and in patients with leucocytospermia it is 1.8 times higher than that in fertile men. At the same time, the concentration of follicle stimulating hormone in patients with astenozoospermia is 2.2 times lower than that in normozoospermic samples, but it is within the physiological norm. The testosterone level in men with oligozoospermia is 1.6 times lower than that in fertile men, but it remains within the physiological norm. It has been found that the inhibition of arginase activity of spermatozoa positively correlates with a decrease in their concentration in the ejaculate of infertile men with oligozoospermia (r = 0.68).