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Critical Reviews™ in Eukaryotic Gene Expression

年間 6 号発行

ISSN 印刷: 1045-4403

ISSN オンライン: 2162-6502

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.6 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.2 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00058 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.33 SJR: 0.345 SNIP: 0.46 CiteScore™:: 2.5 H-Index: 67

Indexed in

Validation of Housekeeping Genes for Gene Expression Profiling in Fish: A Necessity

巻 29, 発行 6, 2019, pp. 565-579
DOI: 10.1615/CritRevEukaryotGeneExpr.2019028964
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要約

Real-time polymerase chain reaction (qPCR) is a reliable tool for gene expression analysis in various organisms. Its use in aquaculture and study of fish physiology is increasing day by day. Relative quantification of mRNA through real-time qPCR requires suitable housekeeping genes/reference genes. Expression of housekeeping genes were thought to be constant in different tissues under various physiological and experimental conditions. In light of the literature reviewed here, it is clear that expression of most of the genes varied in different physiological conditions, under different experimental conditions, and in different gender. Use of housekeeping genes without proper validation may lead to erroneous results and misinterpretation of data. To solve this problem, various types of user-friendly software are now available for validation of housekeeping genes. Moreover, it is recommended to use more than one housekeeping gene for relative quantification. Using the geometric mean of the three most stable genes as a housekeeping gene can also solve this problem to some extent. Therefore, there is a dire need to validate a set of housekeeping genes in the species under study and in various tissues depending on the experimental design to obtain valid and reproducible results.

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  1. Sun Shuaijie, Wang Zhitong, Yuan Dongdong, Ni Mengke, Xu Huifen, Wang Wanliang, Zhang Chi, Chen Meiqun, ZhaXi Lamu, Zhou Jianshe, Li Ming, Selection of stable reference genes for RT–qPCR in Salmo trutta, Aquaculture Reports, 26, 2022. Crossref

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