ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
Journal of Environmental Pathology, Toxicology and Oncology
インパクトファクター: 1.625 5年インパクトファクター: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN 印刷: 0731-8898
ISSN オンライン: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i1.20
pages 11-19

Effect of Ukrain on Cell Survival and Apoptosis in the Androgen-Independent Prostate Cancer Cell Line PC-3

Kavitha Venkatesh
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Tamilnadu, India
Sharmila Govindaraj
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Tamilnadu, India
Arunkumar Ramachandran
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Tamilnadu, India
Senthilkumar Kalimuthu
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Tamilnadu, India
Elumalai Perumal
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Tamilnadu, India
Sivakamasundari Velayutham
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Tamilnadu, India
Arunakaran Jagadeesan
Dr.ALM.PGIBMS

要約

Prostate cancer is the most common malignancy and second leading cause of cancer death in male population in all developing countries. Ukrain is a reaction product of different alkaloids from Chelidonium majus L. (celandine) conjugated with thiophosphoric acid. It has cytotoxic effects on various malignant cells. Cell viability was assessed by MTT method in PC-3 cells after the treatment of Ukrain. The IC50 value was observed in 10 μg concentration of Ukrain. Bax, Bad and FasL mRNA expressions were analysed by RT-PCR and protein expressions of p- Akt, Bcl-2 and caspase 10 were analysed by western blot method. Nuclear staining was detected by DAPI staining. Ukrain significantly increased the proapoptotic mRNA expressions of Bad, Bax and FasL. Ukrain decreased the cell survival protein p-Akt and also antiapoptotic protein Bcl-2. Ukrain increased the protein levels of cleaved PARP and caspases-10. This study suggests that Ukrain decreases the cell survival of androgen independent prostate cancer cells and induces apoptosis, which highlights the importance of Ukrain as a therapeutic drug for the androgen independent prostate cancer treatment.


Articles with similar content:

10H-3,6-Diazaphenothiazine-Induced, Caspases-Mediated Mitochondrial-Dependent Apoptosis on HepG2 Hepatocellular Carcinoma Cells and Suppressed Cancer Cells Invasion via Inhibiting Mitochondrial Thioredoxin Reductase Enzymatic Activities
Journal of Environmental Pathology, Toxicology and Oncology, Vol.38, 2019, issue 4
Kok Pian Ang, Kah Kooi Ooi, Jinhui Zhang, Fa Fang, Qinwen Tai, Yi Gao, Yiqiang Zhu, Yuanxi Wang, Junsheng Kang, Feng Gao, Feng Zhong, Liquan Cai
Role of Hydroalcoholic Extract of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Facilitating Cellular Acclimatization in a Low-Oxygen Microenvironment
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 5
Kshipra Misra, Praveen Koganti, Manish Sharma, Rajkumar Tulsawani, Purva Sharma, Shivani Arora
Effect of Amentoflavone, a Phenolic Component From Biophytum sensitivum, on Cell Cycling and Apoptosis of B16F-10 Melanoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 4
K.S. Siveen , Girija Kuttan
Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
P. Pratheeshkumar, Girija Kuttan
Yin Yang 1 Is a Tumor Immune-Suppressor Gene Product
Forum on Immunopathological Diseases and Therapeutics, Vol.1, 2010, issue 1-2
Stavroula Baritaki