年間 4 号発行
ISSN 印刷: 0731-8898
ISSN オンライン: 2162-6537
Indexed in
Silence of PTEN in Colorectal Cancer Cells Via siRNA Inhibits Cell Growth
要約
Colorectal cancer is one of the most commonly diagnosed cancers and is a leading cause of cancerrelated death worldwide. In this study, we aimed to examine the expression of PTEN in human colorectal cancer cell lines HCT-8 and to further investigate the functions of PTEN in colorectal cancer cells. Therefore, we established stably transfected HCT-8 cell lines expressing siRNA targeting the PTEN gene. Cell proliferation and cell migration of the siPTEN cells were characterized by the CCK-8 assay and the Transwell assay, respectively. Our results show that constitutive knockdown of the PTEN gene in siPTEN cells significantly promoted cell proliferation and migration. These results suggest that PTEN may play important roles in colorectal cancer cell proliferation and migration.
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KAWANO MASANORI, TANAKA KAZUHIRO, ITONAGA ICHIRO, IWASAKI TATSUYA, TSUMURA HIROSHI, MicroRNA-301a promotes cell proliferation via PTEN targeting in Ewing's sarcoma cells, International Journal of Oncology, 48, 4, 2016. Crossref
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Jia Zeming, Peng Jian, Yang Zhi, Chen Jie, Liu Ling, Luo Dongren, He Panxiang, Long non-coding RNA TP73‑AS1 promotes colorectal cancer proliferation by acting as a ceRNA for miR‑103 to regulate PTEN expression, Gene, 685, 2019. Crossref