RT Journal Article
ID 61de5e7d637e1a83
A1 Altinay, Burcu
A1 Karaduman, Ayse Betul
A1 Gursu, Bukay Yenice
A1 YamaƧ, Mustafa
T1 Enhanced Bioactive Exopolysaccharide Production by Mossy Maze Polypore, Cerrena unicolor (Higher Basidiomycetes) in Submerged Culture Conditions
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2015
FD 2015-10-09
VO 17
IS 7
SP 639
OP 648
K1 medicinal mushrooms
K1 Cerrena unicolor
K1 exopolysaccharide
K1 hypoglycemic agent
K1 optimization
K1 stirred tank reactor
AB In this study, the culture requirements of the Cerrena unicolor OBCC 5005 strain were determined to optimize bioactive exopolysaccharide production in submerged culture. The effects of initial medium pH, carbon and nitrogen sources, inoculum age and amount, and mineral source on exopolysaccharide and mycelial biomass production by the C. unicolor OBCC 5005 strain were studied using a one-factor-at-a-time method. The highest exopolysaccharide production was obtained when culture parameters were used as initial medium pH: 5.5, 5% sucrose, 5% mycological peptone, and 5% of 4-day inoculants in the presence of 5 mM Fe2+. Optimized culture conditions at a flask scale were applied to a 3-L stirred tank reactor. As a result, 7.92 g/L and 7.34 g/L maximum exopolysaccharide production in optimized conditions at flask and stirred-tank reactor scales were achieved, respectively. The present study is the first to prove that C. unicolor can yield high bioactive exopolysaccharide production at flask and stirred-tank reactor scales.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,05ab5a7e40e7ba9a,61de5e7d637e1a83.html