RT Journal Article
ID 5e7efbfd2e825d75
A1 Zhu, Lina
A1 Tang, Qingjiu
A1 Zhou, Shuai
A1 Liu, Yanfang
A1 Zhang, Zhong
A1 Gao, Xinhua
A1 Wang, Shiping
A1 Wang, Zhaolong
T1 Isolation and Purification of a Polysaccharide from the Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes) Fruit Bodies and Its Immunomodulation of RAW 264.7 Macrophages
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2014
FD 2014-06-04
VO 16
IS 3
SP 247
OP 257
K1 medicinal mushrooms
K1 Cordyceps militaris
K1 polysaccharide
K1 monosaccharide composition
K1 immunostimulating activity
AB A novel polysaccharide (CP2-S) was purified from Cordyceps militaris fruit bodies by hot water extraction, ethanol precipitation, DEAE-Sepharose Fast Flow and Sephacryl S-400 high-resolution chromatography. The polysaccharide had a molecular weight of 5.938 × 106 g/mol and was mainly composed of glucose. CP2-S had carbohydrate content estimated to be 100% using the phenol-sulfuric acid method. Immunostimulating experiments in vitro indicated that CP2-S could stimulate nitric oxide production, phagocytosis, respiratory burst activity, and secretion of interleukin-1β and interleukin-2 of macrophages, suggesting that this water-soluble polysaccharide from the fruit body of C. militaris is a natural immunostimulating polysaccharide with potential for further application.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,23d62e9b62d52918,5e7efbfd2e825d75.html