RT Journal Article
ID 5b63195218af2138
A1 Fu, Shao Chun
A1 Zhang, Mei Yan
A1 Shang, Xiaodong
A1 Chen, Ming-Jie
A1 Tan, Qi
T1 An Axenic Culture System for Fruiting Body Formation by an Edible Bolete Phylogenetically related to Culinary-Medicinal Penny Bun Mushroom, Boletus edulis Bull.:Fr. Strains from China
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2011
FD 2011-10-07
VO 13
IS 4
SP 387
OP 395
K1 culinary-medicinal mushrooms
K1 Boletus spp.
K1 ectomycorrhizal mushroom
K1 primordium induction
K1 fruiting body formation
K1 axenic culture
K1 artificial cultivation
AB The ability of two freshly isolated Boletus stains to fruit under axenic conditions was tested using different solid and liquid nutrient media. One strain (YNCX04) produced numerous primordia from which fruiting bodies, 12 mm and 10 mm in length, with grey, convex pilei, and yellow-white, clavate stipes developed between 15 and 30 d after inoculation of fungal mycelium onto a solid medium consisting of mineral salts, thiamine, glucose, potato, an extract of Cunninghamia lanceolata root, and agar. The other strain (YNB200) produced numerous primordia but no sporophores. Strain YNCX04 lost the ability to form fruiting bodies in axenic culture 6 mo after initial isolation but retained the ability to form primordia for up to 18 mo. Based on internal transcribed spacer sequencing data, strains YNB200 and YNCX04 formed a sub-cluster together with four previously designated Boletus edulis strains from China. Phylogenetic analysis placed the Chinese strains closer to B. aestivalis than to European and North American strains of B. edulis, although a 29-bp fragment specific to all the B. aestivalis strains was absent from all the Chinese strains. Furthermore, partial 18S rDNA sequences from strains YNB200 and YNCX04 exhibited 98% similarity with an 18S rDNA sequence from B. edulis strain Be3. Further molecular studies are indicated to more accurately establish the taxonomic positions of F3 and F4-3, as well as the Chinese strains designated as B. edulis.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7157a84928f91612,5b63195218af2138.html