%0 Journal Article %A Guo, Xuefang %A Wang, Xiaoli %A Liu, Min %A Chen, Lei %A Chanda, Warren %A Pahiar, Arshad Ahmed %A Li, Xingyun %A Zhang, Wei %A Ning, Anhong %A Huang, Min %A Cao, Jing %A Zhong, Mintao %D 2018 %I Begell House %K Lentinus edodes, transcriptome, Latcripin-4 RCC1 and ANK domain protein, antitumor, medicinal mushrooms %N 12 %P 1163-1172 %R 10.1615/IntJMedMushrooms.2018028430 %T Expression and Antitumor Function of Latcripin-4 RCC1 and ANK Domain Protein on HepG2 from the Shiitake Medicinal Mushroom, Lentinus edodes C91–3 (Agaricomycetes), Transcriptome %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2c6ab3993645ca9e,5b1694f16d976987.html %V 20 %X The recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91–3 transcriptome by bioinformatics. Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome. The RCC1 and ANK domains from the full-length gene were selected and inserted into the expression vector pET-32a (+) and expressed in Escherichia coli Rosetta-gami (DE3). Western blotting indicated that the protein was expressed successfully. The biological function of Latcripin-4 RCC1 and ANK domain protein on HepG2 cells was studied with the CCK-8 assay. All results demonstrated that Latcripin-4 RCC1 and ANK domain protein can inhibit the growth of human HepG2 liver cancer cells, which brings new insights to identifying antitumor proteins from medicinal food for cancer therapy. %8 2018-11-30