%0 Journal Article
%A Guo, Xuefang
%A Wang, Xiaoli
%A Liu, Min
%A Chen, Lei
%A Chanda, Warren
%A Pahiar, Arshad Ahmed
%A Li, Xingyun
%A Zhang, Wei
%A Ning, Anhong
%A Huang, Min
%A Cao, Jing
%A Zhong, Mintao
%D 2018
%I Begell House
%K Lentinus edodes, transcriptome, Latcripin-4 RCC1 and ANK domain protein, antitumor, medicinal mushrooms
%N 12
%P 1163-1172
%R 10.1615/IntJMedMushrooms.2018028430
%T Expression and Antitumor Function of Latcripin-4 RCC1 and ANK Domain Protein on HepG2 from the Shiitake Medicinal Mushroom, Lentinus edodes C91–3 (Agaricomycetes), Transcriptome
%U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2c6ab3993645ca9e,5b1694f16d976987.html
%V 20
%X The recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91–3 transcriptome by bioinformatics.
Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome. The RCC1 and ANK domains from the full-length gene were selected and inserted into the expression vector pET-32a (+) and expressed in Escherichia coli Rosetta-gami (DE3). Western blotting indicated that the protein was expressed successfully. The biological function of Latcripin-4 RCC1 and ANK domain protein on HepG2 cells was studied with the CCK-8 assay. All results demonstrated that Latcripin-4 RCC1 and ANK domain protein can inhibit the growth of human HepG2 liver cancer cells, which brings new insights to identifying antitumor proteins from medicinal food for cancer therapy.
%8 2018-11-30