RT Journal Article
ID 5283845876ccedf8
A1 Liu, Qing 
A1 Wang, Fen 
A1 Liu, Kuanbo
A1 Dong, Caihong
T1 Influence of Strain Preservation Methods on Fruiting Body Growth and Metabolite Production by the Medicinal Mushroom <i>Cordyceps militaris</i> (Ascomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-10-15
VO 20
IS 10
SP 1003
OP 1011
K1 <i>Cordyceps militaris</i>
K1 degeneration
K1 lyophilization
K1 medicinal mushrooms
K1 preservation
K1 sterile water
AB <i>Cordyceps militaris</i> is a highly valued edible and medicinal fungus because of its production of various metabolites including adenosine, cordycepin, and N<sup>6</sup>-(2-hydroxyethyl)-adenosine. Fruiting bodies of this fungus have been used successfully in industrial production and widely as a substitute for <i>Ophiocordyceps sinensis</i> (syn. <i>C. sinensis</i>) in traditional Chinese medicine and health supplements. Strain degeneration occurs with high frequency during the subculturing and preservation of <i>C. militaris </i>strains, which leads to significant losses during industrial production. In this study, we evaluated the effects of different strain preservation methods on fruiting body growth and metabolite production. We found that strain degeneration affects not only fruiting body differentiation but also metabolite production, and suitable preservation methods can avoid degeneration. Preservation in sterile water has a similar effect as cryopreservation in liquid nitrogen at -196&deg;C with regard to maintaining the characteristics of <i>C. militaris </i>strains for at least 1 year, and it is a practical and satisfactory method for preserving <i>C. militaris</i> strains that can be used in factories. Ultracold freezing at -80&deg;C is not suitable for this fungus. Lyophilization, which causes <i>C. militaris</i> strains to retain their inherent characteristics and avoid degeneration, is suitable for long-term preservation (at least 4 years). This study provides practical preservation methods for <i>C. militaris</i> strains over the short and long term and will be helpful to achieve stable and superior-quality production of <i>C. militaris</i> fruiting bodies.

PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2e8a1fed297cf479,5283845876ccedf8.html