RT Journal Article
ID 49d6b53600baaace
A1 Ganie, Showkat Ahmad
A1 Dar, Tanveer Ali
A1 Zargar, Bilal
A1 Hamid, Rabia
A1 Zargar, Ovais
A1 Dar, Parvaiz Ahmad
A1 Abeer, Shayaq Ul
A1 Masood, Akbar
A1 Amin, Shajrul
A1 Zargar, Mohammad Afzal
T1 Antioxidant and Hepatoprotective Effects of Crataegus songarica Methanol Extract
JF Journal of Environmental Pathology, Toxicology and Oncology
JO JEP(T)
YR 2014
FD 2014-05-09
VO 33
IS 2
SP 131
OP 143
K1 hepatoprotective effect
K1 Crataegus songarica extract
K1 liver injury
K1 chronic CCl4 paracetamol
K1 oxidative stress
AB The protective activity of the methanolic extract of the Crataegus songarica leaves was investigated against CCl4- and paracetamol-induced liver damage. On folklore levels, this plant is popularly used to treat various toxicological diseases. We evaluated both in vitro and ex vivo antioxidant activity of C. songarica. At higher concentration of plant extract (700 µg/ml), 88.106% inhibition on DPPH radical scavenging activity was observed and reducing power of extract was increased in a concentration-dependent manner. We also observed its inhibition on Fe2+/ascorbic acid-induced lipid peroxidation on rat liver microsomes in vitro. In addition, C. songarica extract exhibited antioxidant effects on calf thymus DNA damage induced by Fenton reaction. Hepatotoxicity was induced by challenging the animals with CCl4 (1 ml/kg body weight, i.p.) and paracetamol (500 mg/kg body weight) and the extract was administered at three concentrations (100, 200, and 300 mg/kg body weight). Hepatoprotection was evaluated by determining the activities of liver function marker enzymes and antioxidant status of liver. Administration of CCl4 elevated the levels of liver function enzymes, SGOT, SGPT, and LDH. We also observed a dramatic increase in ALT, AST, bilirubin, and alkaline phosphatase levels in rats administered 500 mg/kg body weight of paracetamol. Decreased antioxidant defense system as glutathione (GSH), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione-S-transferase (GST), and superoxide dismutase (SOD) were observed in rats treated with CCl4 and paracetamol. Pretreatment with the extract decreased the elevated serum GOT, GPT, LDH, bilirubin, and alkaline phosphatase activities and increased the antioxidant enzymes in a dose-dependent manner. Therefore, C. songarica methanol extract may be an effective hepatic protective agent and viable candidate for treating hepatic disorders and other oxidative stress-related diseases.
PB Begell House
LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,249005dc454eb8de,49d6b53600baaace.html