RT Journal Article
ID 3ea896c87bb716d7
A1 Barton, Annemarie
A1 Wende, Kristian
A1 Bundscherer, Lena
A1 Hasse, Sybille
A1 Schmidt, Anke
A1 Bekeschus, Sander
A1 Weltmann, Klaus-Dieter
A1 Lindequist, Ulrike
A1 Masur, Kai
T1 Nonthermal Plasma Increases Expression of Wound Healing Related Genes in a Keratinocyte Cell Line
JF Plasma Medicine
JO PMED
YR 2013
FD 2014-10-01
VO 3
IS 1-2
SP 125
OP 136
K1 nonthermal plasma
K1 keratinocytes
K1 wound healing
K1 gene expression
K1 HaCaT cells
K1 plasma medicine
AB The application of physical plasma in medicine has great potential in wound healing. Due to the generation of reactive oxygen and nitrogen species (ROS, RNS), emission of UV radiation and the generated electric fields can be used to stimulate epithelial and immune cells. To understand the processes on a molecular level the human keratinocyte cell line HaCaT was treated with a nonthermal atmospheric pressure argon plasma jet (kinpen). Subsequently, cellular RNA was isolated to conduct a quantitative polymerase chain reaction (qPCR) to monitor the magnitude of translation of genes related to wound healing. Plasma treatment induced an upregulation of vascular endothelial growth factor-a (VEGF-A), heparin-binding epidermal growth factor (EGF)-like growth factor (HBEGF), granulocyte macrophage colony-stimulating factor (GM-CSF), prostaglandin-endoperoxide synthase 2 (PTGS2) and interleukin-6 (IL-6) at the mRNA levels. This is a very promising result as the corresponding proteins are likely to be secreted and promote the wound healing process. Therefore plasma potentially induces the secretion of certain cytokines and growth factors, and hence, it could be the stimulus which is necessary to induce chronic wounds to heal.
PB Begell House
LK https://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,4eec539e3367d43b,3ea896c87bb716d7.html