RT Journal Article ID 3cc4cd700e235bf7 A1 Villares, Ana T1 Isolation and Characterization of a Glucan-Type Polysaccharide from the Red Pine Mushroom, Lactarius deliciosus (Higher Basidiomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2013 FD 2013-11-15 VO 15 IS 6 SP 583 OP 589 K1 medicinal and edible mushrooms K1 Lactarius deliciosus K1 red pine mushroom K1 polysaccharides K1 glucan K1 NMR. AB A novel glucan-type polysaccharide has been isolated from the fruiting bodies of the edible mushroom Lactarius deliciosus. Two successive extractions (cold aqueous extraction at 25°C and hot aqueous extraction at 100°C) were performed, and the same polysaccharide was obtained in both fractions. The purity of the polysaccharide was evaluated by size exclusion chromatography. The SEC chromatogram showed a unique peak with a molecular weight of approximately 150 kDa. Analysis of the chain composition revealed that the polysaccharide was composed of glucose. Methylation analysis and NMR experiments showed that the glucan-type carbohydrate contained a main chain consisting of α-(1→3)-Glcp units with α-(1→4)-linked branches every sixth glucose residue. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,034eeb045436a171,3cc4cd700e235bf7.html