RT Journal Article
ID 3cc4cd700e235bf7
A1 Villares, Ana
T1 Isolation and Characterization of a Glucan-Type Polysaccharide from the Red Pine Mushroom, Lactarius deliciosus (Higher Basidiomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2013
FD 2013-11-15
VO 15
IS 6
SP 583
OP 589
K1 medicinal and edible mushrooms
K1 Lactarius deliciosus
K1 red pine mushroom
K1 polysaccharides
K1 glucan
K1 NMR.
AB A novel glucan-type polysaccharide has been isolated from the fruiting bodies of the edible mushroom Lactarius deliciosus. Two successive extractions (cold aqueous extraction at 25°C and hot aqueous extraction at 100°C) were performed, and the same polysaccharide was obtained in both fractions. The purity of the polysaccharide was evaluated by size exclusion chromatography. The SEC chromatogram showed a unique peak with a molecular weight of approximately 150 kDa. Analysis of the chain composition revealed that the polysaccharide was composed of glucose. Methylation analysis and NMR experiments showed that the glucan-type carbohydrate contained a main chain consisting of α-(1→3)-Glcp units with α-(1→4)-linked branches every sixth glucose residue.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,034eeb045436a171,3cc4cd700e235bf7.html