RT Journal Article ID 2d9a2968652b06f5 A1 Maslov, Vitally Y. A1 Veselovsky, Mycola S. A1 Moskaluk, Anastasiya A. A1 Myasoedov, Nikolai F. A1 Shram, Stanislav I. A1 Fedulova, Svetlana A. T1 Peptide Pro-Gly-Pro Stabilzes Electrophysiological Characteristics of Cultured Hippocampal Neurones during Excitotoxic Damage JF International Journal of Physiology and Pathophysiology JO IJPP YR 2015 FD 2015-07-02 VO 6 IS 2 SP 109 OP 118 K1 neurons culturing K1 excitotoxicity K1 neuroprotective action K1 Pro-Gly-Pro peptide AB Effect of culturing with Pro-Gly-Pro peptide on electrophysiological characteristics of hippocampal neurons was studied using glutamate excitotoxicity model (excitotoxic damage was induced by 100 µM glutamate application during 5 min). It has been found that negative changes in neurons cultured with 10 µM Pro-Gly-Pro were less prominent compared to the control ones. In particular, culturing with Pro-Gly-Pro significantly affected the resting potential (-55 ± 4 mV in the control,-29 ± 6 mV after glutamate application, and-38 ± 5 mV after glutamate application at culturing with peptide), action potential amplitude (91 ± 4; 65 ± 5; 84 ± 5 mV), duration (4.3 ± 0.4; 9.5 ± 1.6; 5.2 ± 0.7 ms), depolarization rate (56 [38, 84]; 27 [21, 35]; 46 [28, 62] mV/ms) and repolarization rate (-29 [-38,-27];-20 [-21,-18];-29 [-33,-22] mV/ms). The data obtained suggest that Pro-Gly-Pro peptide exhibits its neuroprotective properties at the level of basic electrophysiological characteristics, although appropriate cellular mechanisms require further investigations. PB Begell House LK https://www.dl.begellhouse.com/journals/6ec4ba27650016b1,2c4341610e627acb,2d9a2968652b06f5.html