RT Journal Article ID 10c3a2a60c6d3d0e A1 Cong, Wei-Ran A1 Gong, Zhen-Hua A1 Shi, Dan-Dan A1 Guo, Hui A1 Zhou, Xuan-Wei T1 Cloning of Mating-Type Gene MAT1-1 from the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes) Using TAIL-PCR Technology JF International Journal of Medicinal Mushrooms JO IJM YR 2014 FD 2014-09-12 VO 16 IS 5 SP 449 OP 461 K1 medicinal mushrooms K1 Cordyceps militaris K1 TAIL-PCR K1 mating-type K1 MAT1-1-1 gene K1 bioinformation AB Cordyceps militaris and Ophiocordyceps sinensis (syn. Cordyceps sinensis), 2 well-known traditional Chinese medicines, contain the same bioactive components and share a similar developmental process. In this study, one C. militaris strain preserved in our laboratory was proven to be a MAT1 mating-type strain using a polymerase chain reaction−based mating-type assay. A 5000-bp nucleotide sequence of the mating-type MAT1-1 from C. militaris was amplified by thermal asymmetric interlaced polymerase chain reaction, but genes within the mating-type MAT1-2 remain undetectable. Sequence analysis shows that the mating-type gene MAT1-1 idiomorph contains 2 genes, MAT1-1-1 and MAT1-1-2. The MAT1-1-1 gene consists of 1480-bp nucleotides that encode 456 amino acids and contain the conserved a-box domain interrupted by 2 introns; the MAT1-1-2 gene consists of 1066 nucleotides that encode 377 amino acids interrupted by one intron. The intervening distance between MAT1-1-1 and MAT1-1-2 is 778 bp. The C. militaris MAT1-1 idiomorph organization is the same as that of Cordyceps takaomontana. The MAT1-1 mating-type idiomorph of both Cordyceps species lacks the MAT1-1-3 gene, which is typically present in Pyrenomycetes. These studies provide some insights for further study of the morphological development of C. militaris and will eventually benefit the domestication of O. sinensis. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,266d4152107fca7a,10c3a2a60c6d3d0e.html