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International Journal on Algae
SJR: 0.216 SNIP: 0.322 CiteScore™: 0.4

ISSN Print: 1521-9429
ISSN Online: 1940-4328

International Journal on Algae

DOI: 10.1615/InterJAlgae.v6.i2.50
pages 151-157

Heavy metal toxicity on nitrate reductase activity of free and immobilized algal cells

Mamta Awasthi
Department of Zoology, Arunachal University, Itanagar, 791 112, India
Debanghshu Narayan Das
Arunachal University, Itanagar 791 112, India

ABSTRACT

Nitrate assimilation is the principal route for plants and algae to acquire inorganic nitrogen. Nitrate reductase is a multi-enzyme complex bound in the cell membrane in both active and inactive forms. Nitrate reductase is the limiting factor when considering the growth and protein production of algae. Heavy metals interacted negatively with the nitrate reductase (NR) enzyme activity of Chlorella vulgaris Beijer. Nickel was found to be more toxic compared to zinc and cadmium. A highly significant increase in NR activity was observed in the immobilized cells over free cells when supplemented with Ni.