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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v4.i3.60
4 pages

Quantitative Method Measurement of Flammulin Concentration with Immunoassay

Hua Zhang
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Youchun Zhao
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Hanxing Zhang
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Yongjun Feng
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Changkai Zhang
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China

ABSTRACT

Flammulin purified from the aqueous extract of basidiomes of edible and medicinal mushroom Flammulina velutipes was completely emulsified with Freund adjuvant, then injected into New Zealand white rabbits. After several immunity enhancements, these animals were bled and serum was separated. Double diffusion showed that the antiserum could react with flammulin and form the precipitate cycle in agarose gel with valence of 1:16. According to the result of simple agar diffusion of flammulin with serum on agarose gel plate, a group of standard curves, fitting the reaction results of flammulin at different concentrations, were established, which can be used in the quantitative measurement of flammulin concentration.


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