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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i5.70
pages 457-465

A Validated Reverse-Phase HPLC Method for Quantitative Determination of Ganoderic Acids A and B in Cultivated Strains of Ganoderma spp. (Agaricomycetes) Indigenous to India

M. Ramakrishna
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
D. Rajesh Babu
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
S. S. Veena
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Meera Pandey
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Nageswara Rao
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India

ABSTRACT

Twenty isolates of Ganoderma spp. indigenous to India were used in this study. A reverse-phase high-performance liquid chromatography (HPLC) method was used to quantify 2 of the most bioactive triterpenes, ganoderic acid A (GAA) and ganoderic acid B (GAB), among the cultivated Ganoderma spp. The HPLC analysis was performed on an Agilent 1260 Infinity HPLC system with a Zorbax C18 column, using gradient elution of acetonitrile and 0.1% acetic acid. The detection wavelength was set at 254 nm, with a flow rate of 0.6 mL/min. The method was validated according to the guidelines of International Conference on Harmonisation and produced satisfactory results. The amount of GAA varied from 827.50 to 2010.36 µg/g, whereas GAB varied from 16.64 to 916.89 µg/g. The developed method is simple, specific, accurate, and precise, and can be useful for qualitative and quantitative evaluation of ganoderic acids.


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