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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v10.i4.90
pages 361-368

Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)

George G. Songulashvili
Institute of Evolution, Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, 31905 Israel; and Durmishidze Institute of Biochemistry and Biotechnology,0159 Tbilisi, Georgia
Vladimir I. Elisashvili
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia
Eviatar D. Nevo
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, Institute of Evolution, University of Haifa, 199 Abba Khousi Ave., Mt. Carmel, Haifa 3498838, Israel
Yitzhak Hadar
Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel

ABSTRACT

The Ganoderma lucidum strain 447 was cultivated in a 10-L fermentor. We used the ethanol-production residue (by wheat) (REP) as the growth substrate and Cu as an inductor. Ganoderma lucidum was grown for 8 days before laccase reached the highest yield (188,600 U L−1). The purification enzyme appeared as two-laccase isozyme bands on SDS-PAGE. The molecular masses were 43 and 56 kDa by SDS-PAGE. The pH optimum for 2,2'-azino-bis-[3-ethyltiazoline-6-sulfonate] (ABTS) oxidation was 3 in citric/acetic buffers. In this study, the optimum temperature for laccase activity was determined to be 30°C. The kinetic of laccase was experimented on using 12 phenolic substrates. The lowest Km values (0.0048 and 0.005 mM) were found for syringaldazine and ABTS, respectively.


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