Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v4.i4.40
7 pages

Fractionation and Characterization of Water-Soluble Polysaccharides from Culinary-Medicinal Mushroom, Agaricus blаzеi Murrill (Agaricomycetideae) Fruit Body

Lin Yuexin
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China
Ye Zhuqiu
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China
Huang Yanyan
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China
Xie Hualing
Bioengineering College, Fujian Teachers University, Fuzhou 350007, People's Republic of China

ABSTRACT

Three groups of water-soluble polysaccharides, AB-I, AB-II-b, and AB-III-b, were obtained from Agaricus blazei fruit body by hot-water extraction, ethanol precipitation, DEAE-cellulose chroniatography, and Sephadex G-100 gel filtration. HPLC examination showed that they were purified into pure preparations. The molecular weight, monosaccharide constituent, and glucosidic bond of the polysaccharides were tested by employing Sepharose CL-4B, GC, and IR. The results were as follows: molecular weight: AB-I, 21 ґ 104, AB-II-b, 34 ґ 106, AB-III-b, 6 ґ 104; monosaccharide composition: AB-I, Gal:Man:Glc (1.43:2.02:5.57), AB-II-b, Rha:Gal:Man:Glc (1.00:1.26:2.75:4.26), AB-III-b, Rha:Xyl:Gal:Man:Glc (1.49:0.37:0.84:1.86:4.05). IR examination showed that the glucosidic bond of the three polysaccharides were b-pyran type. The polysaccharides contained a protein constituent.


Articles with similar content:

Immune-Competent Polysaccharides from the Submerged Cultured Mycelium of Culinary—Medicinal Mushroom Lentinus strigellus Berk. et Curt. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 1
Ping Lai, Yuexin Lin, Hualing Xie, Yanyan Huang
Triterpenes and Sterols from Ganoderma australe (Fr.) Pat. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.2, 2000, issue 4
Nelson Biacchi, Jr., Alexandra Lehmkuhl Gerber, Artur Smania, Jr., Franco Delie Monache, Elza de Fatima Albino Smania
Extraction of Conformationally Stable (1®6)-Branched (1®3)-b-Glucans from Premixed Edible Mushroom Powders by Cold Alkaline Solution
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 3
Manabu Kanai, Yoshiyuki Adachi, Toshiro Yadomae, Masaji Sawai, Susumu Matsui
The Isolation, Structure, and Applications of the Exocellular Heteropolysaccharide Glucuronoxylomannan Produced by Yellow Brain Mushroom Tremella mesenterica Ritz.:Fr. (Heterobasidiomycetes)
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 4
Bent O. Petersen, Jens O. Duus, Evgeny Vinogradov
Mycelium Macromorphology and Growth Characteristics of Culinary-Medicinal Mushroom Flammulina velutipes (W.Curt.: Fr.) Singer
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Carmen Z. Sakeyan, Susanna M. Badalyan