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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i4.90
pages 387-395

An Axenic Culture System for Fruiting Body Formation by an Edible Bolete Phylogenetically related to Culinary-Medicinal Penny Bun Mushroom, Boletus edulis Bull.:Fr. Strains from China

Shao Chun Fu
National Eng.Research Center of Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization, Chinese Ministry of Agriculture; Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi
Mei Yan Zhang
National Eng.Research Center of Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization, Chinese Ministry of Agriculture; Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi
Xiaodong Shang
National Eng.Research Center of Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization, Chinese Ministry of Agriculture; Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi
Ming-Jie Chen
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Agricultural Genetics and Breeding, Ministry of Agriculture, National Engineering Research Center for Edible Fungi, National R&D Center for Edible Fungi Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China
Qi Tan
National Engineering Research Center of Edible Fungi, Ministry of Science and Technology (MOST), Key Laboratory of Edible Fungal Resources and Utilization (South), Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

ABSTRACT

The ability of two freshly isolated Boletus stains to fruit under axenic conditions was tested using different solid and liquid nutrient media. One strain (YNCX04) produced numerous primordia from which fruiting bodies, 12 mm and 10 mm in length, with grey, convex pilei, and yellow-white, clavate stipes developed between 15 and 30 d after inoculation of fungal mycelium onto a solid medium consisting of mineral salts, thiamine, glucose, potato, an extract of Cunninghamia lanceolata root, and agar. The other strain (YNB200) produced numerous primordia but no sporophores. Strain YNCX04 lost the ability to form fruiting bodies in axenic culture 6 mo after initial isolation but retained the ability to form primordia for up to 18 mo. Based on internal transcribed spacer sequencing data, strains YNB200 and YNCX04 formed a sub-cluster together with four previously designated Boletus edulis strains from China. Phylogenetic analysis placed the Chinese strains closer to B. aestivalis than to European and North American strains of B. edulis, although a 29-bp fragment specific to all the B. aestivalis strains was absent from all the Chinese strains. Furthermore, partial 18S rDNA sequences from strains YNB200 and YNCX04 exhibited 98% similarity with an 18S rDNA sequence from B. edulis strain Be3. Further molecular studies are indicated to more accurately establish the taxonomic positions of F3 and F4-3, as well as the Chinese strains designated as B. edulis.