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International Journal of Medicinal Mushrooms

Impact factor: 1.123

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i4.70
pages 369-375

Molecular Identification and Characterization of the Edible and Medicinal Morchellaceae Germplasm Collection of "Mulch Morels"

Vladan Ondrej
Laboratory of Plant Tissue Cultures, Department of Botany, Faculty of Science, Palacky University, Slechtitelu 11, Olomouc 783 71, Czech Republic
Pavel Havranek
Laboratory of Plant Tissue Cultures, Department of Botany, Faculty of Science, Palacky University, Slechtitelu 11, Olomouc 783 71, Czech Republic
Miloslav Kitner
Laboratory of Plant Tissue Cultures, Department of Botany, Faculty of Science, Palacky University, Slechtitelu 11, Olomouc 783 71, Czech Republic
Pavla Nemcova
Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, Olomouc, 77200, Czech Republic

ABSTRACT

The accessions of the morel (Morchellaceae, Ascomycota) germplasm collection were genetically analyzed, in order to determine both their inter- and intraspecific relationships. This was done as a starting point for cultivation experiments, as well as to provide a genetic description of invasive morel populations linked to mulched garden patches, as compared with outdoor morels. The phylogenetic data, which was based on the internal transcribed spacer (ITS) sequences and supported by amplified fragment length polymorphism (AFLP) analyses, divided the germplasm isolates and accessions from the sequence database into three groups of yellow morels, and three groups of black morels, involving a remarkable monotypic genus of half-free morels (Mitrophora semilibera), the groups Morchella conica and M. angusticeps. Both Morchella groups include morel samples that use mulch bark as a vector for their spread across gardens in various locations in the Czech Republic. The AFLP analysis supported the ITS-based phylogenetic data and determined the intraspecific genetic profile of these, as a rule, almost entirely unstudied isolates.