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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i4.20
pages 327-333

Chemical Screening and Identification of High Cordycepin Containing Cultured Isolate(s) of Medicinal Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Berk.) G.H. Sung et al.

Vinay K. Varshney
Chemistry and Bio-prospecting Division, Forest Research Institute, Dehradun, India-248006
Amit Pandey
Forest Pathology Division, Forest Research Institute, P.O. New Forest, Dehra Dun 248 006, India
Arvind Kumar
Dept. of Mechanical Engineering, Indian Institute of Technology Kanpur Kanpur, Uttar Pradesh, 208016, India
Divyadhara Rathod
Forest Pathology Division, Forest Research Institute, P.O. New Forest, Dehra Dun 248 006, India
Pooja Kannojia
Forest Pathology Division, Forest Research Institute, P.O. New Forest, Dehra Dun 248 006, India

ABSTRACT

Forty isolates of Ophiocordyceps sinensis collected from Himalayan alpine meadows of Uttarakhand, India, and cultivated on Jhangora (Echinochloa crusgalli) grains were screened to identify the isolate(s) of high cordycepin content. The cultured mycelia were extracted with 50% methanol-chloroform and analyzed by HPTLC using chloroform:methanol (6:1 v/v) as mobile phase and densitometry scanning at 263 nm. Cordycepin varied from 0.002% to 0.029% was detected in twenty-one isolates. Compared to natural O. sinensis (0.004%, 0.006%), cordycepin was determined to be enhanced in twelve cultured samples.


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