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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v1.i1.80
pages 93-103

Cultural Study and llludin S Production of Medicinal Mushroom Omphalotus olearius (DC.Fr.) Fay. (Agaricales s.l.) from Israel

Alexander L. Weis
international Myko Biologies, Inc., Texas Research Park, 14785 Omicron Dr., Suite 104, San Antonio, TX, 78245, U.S.A.
Elvira F. Solomko
M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev, Tereshchenkivska Str. 2, GSP, 01601, Ukraine
Asja S. Buchalo
M. G. Kholodny Institute of Botany National Academy of Sciences of Ukraine, Tereshchenovskaya Str., 2, Kiev 01601, Ukraine
Solomon P. Wasser
N.G. Kholodny Institute of Botany, NAS of Ukraine, 2 Tereshchenkovskaya Str., Kiev 01004, Ukraine; International Center for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mount Carmel, Haifa, Israel
Nadezda Yu. Mitropolskaya
M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereschenkivska Str. 2, Kiev, 252601, Ukraine
Andrey Ph. Grigansky
M.G.Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska Str. 2, Kiev, 252001, Ukraine
Elena L. Gorovits
International Myko Biologies, Inc., 14785 Omicron Dr., Suite 104, San Antonio, TX, 78245, USA

ABSTRACT

The morphological and cultural characteristics of vegetative mycelium of 11 investigated strains of Omphalotus olearius (DC.:Fr.) Fay. (Omphalotaceae, Agaricales s.l.) were studied. The hyphae were found to have numerous clamps and formations of arthroconidia and crystals. The growth rate of mycelium (to 7.5 mm/day) on the surface varied depending on media composition. Production of orange-red pigment and the high activity of gelatinase, urease, cellulase, and β-glucosidase was observed in pure cultures. Optimum temperature was developed for growth appeared to be 28 °C, with an initial pH of complex media between 4.0 and 4.5. A methodical scheme for obtaining standard liquid inoculum useful for physiological and biochemical investigations of O. olearius. Illudin S content in liquid broth under submerged cultivation for all strains was determined. Depending on the strain, the content of illudin S was between 0.81 and 3.1 mg/ml. Strains 162, 167 and 169 have been selected for optimization of illudin S production.


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