Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i6.70
pages 561-570

Cytotoxic Activities of Fractions of the Willow Bracket Medicinal Mushroom, Phellinus igniarius (Agaricomycetes), and the Induction of Cell Cycle Arrest and Apoptosis in MGC-803 Cells

Fei-Fei Wang
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P. R. China
Feng Liu
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P. R. China
Chao Shi
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P. R. China
Wei Ma
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P. R. China
Kai-Jin Wang
School of Life Sciences, Anhui University, Hefei, P. R. China
Ning Li
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P. R. China

ABSTRACT

Phellinus igniarius, a perennial medicinal mushroom, has been used in China and other countries of East Asia for the treatment of various diseases, including cancer. The purpose of this study is to evaluate the cytotoxic activities of different fractions of an ethanol extract from Ph. igniarius and to elucidate a possible antitumor mechanism. An ethanol extract of Ph. igniarius was partitioned into a petroleum ether fraction, an ethyl acetate fraction (EAF), an n-butanol fraction, and a water-soluble fraction. The cytotoxic activity of all the fractions was initially screened in a brine shrimp lethality test, then evaluated by the Cell Counting Kit-8 assay against 5 human tumor cell lines: MGC-803, BEL-7402, HeLa, MCF-7, and HCT-116. The cell cycle distribution induced by EAF on MGC-803 cells was analyzed by flow cytometry with propidium iodide staining, and apoptosis was determined using flow cytometry with Annexin V/propidium iodide staining. The results of the brine shrimp lethality test and the Cell Counting Kit-8 assay showed that EAF was the most active fraction, displaying strong inhibitory activity against the MGC-803, BEL-7402, and MCF-7 cancer cell lines. Flow cytometry analysis indicated that EAF could induce S-phase cell cycle arrest in MGC-803 cells and cause apoptosis in a concentration-dependent manner. This study demonstrated that EAF, as the most active fraction of Ph. igniarius, exerted antitumor activity by inducing MGC-803 cell apoptosis via S-phase cell cycle arrest.


Articles with similar content:

Cytotoxic Activities of Funalia trogii (Berk.) Bond. Et. Singer ATCC 200800 Bioactive Extract on HeLa Cells and Fibroblast Cells
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Mehmet A. Mazmanci, Ali Unyayar, Murat Demirbilek, Ozlem Cekic, Melisa Turkoglu, Hatice Atacag, Serpil Unyayar, Emrah A. Erkurt, Ayla Celik
Antiproliferative and Apoptotic Activities of the Medicinal Mushroom Phellinus rimosus (Agaricomycetes) on HCT116 Human Colorectal Carcinoma Cells
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 10
Chakrappilly Radhakrishnan Meera, Kainoor K. Janardhanan, Devarajan Karunagaran
Comparative evaluation of the cytotoxic and apoptotic potential of Poecilocerus pictus and Calotropis gigantea
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
Shiney Mary Peter, Bhagyashree P. Hardikar, Caroline Mathen
Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Maja Kozarski, Anita Klaus, Jovana Vunduk, Leonardus Johannes Lambertus Donatus Van Griensven, Predrag Petrovic, Miomir Niksic, Zeljko Zizak
Antiproliferative Activity of Some Higher Mushrooms from Hungary against Human Cancer Cell Lines
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Attila Vanyolos, Istvan Zupko, Noemi Bozsity, Judit Hohmann, Bernadett Kovacs