Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i1.20
pages 9-20

Cooperative Effect of the Lipopolysaccharide and Culinary-Medicinal Cauliflower Mushroom Sparassis crispa (Wulf.) Fr. (Aphyllophoromycetideae)-Derived β-Glucan on Inflammatory Cytokine Secretion by the Murine Macrophage Cell Line

Sung In Kim
Department of Life Sciences, University of Seoul, Seoul 130-743, Korea
Hyuk Gu Park
Hana Natural Substance Institute, Hanabiotech Ltd., Yeonchon, Gyonggi 486-860, Korea
Gye Hyung Cho
Department of Life Sciences, University of Seoul, Seoul 130-743, Korea
In Soo Ko
Hana Natural Substance Institute, Hanabiotech Ltd., Yeonchon, Gyonggi 486-860, Korea
Ha Won Kim
Department of Life Sciences, University of Seoul Dongdaemun-gu, Jennong-dong 90, Seoul 130-743, South Korea

ABSTRACT

β-Glucans act as pathogen-associated molecular patterns (PAMPs), which can bind to the pattern recognition receptors (PRRs) on a macrophage or dendritic cell. Many kinds of mushroom β-glucans act as PAMPs and can bind to dectin-1 as one of the PRRs. High-molecular-weight β-glucans or intestinal normal microbiota can be transported through lumen epithelial cells, resulting in the stimulation of a macrophage in Peyer's patch. To study the involvement of TLR4 in the stimulation of dectin-1, the RAW264.7 macrophage cell line was stimulated using the β-glucan of Sparassis crispa (BGS) as well as the lipopolysaccharide (LPS) as a TLR4 ligand. The induction of IL-6 and the inducible nitric oxide synthase (iNOS) mRNAs was increased in a BGS and TNF-α concentration-dependent pattern. The increases of the TNF-α and IL-6 secretions after their treatment with 10 μg/mL BGS were found to be 204- and 420-fold, which are much higher, compared to those of the control. Moreover, the secretions of such cytokines were further increased by the presence of LPS, indicating the involvement of TLR4 in the signaling by dectin-1 with BGS. The secretion of nitric oxide (NO) was increased by BGS. These results show that BGS stimulated innate immunity by activating the macrophages through the induction of the dectin-1 receptor and the inflammatory cytokines. The secretion of inflammatory cytokines was influenced by TLR4 stimulation.


Articles with similar content:

β-Glucan of Ganoderma lucidum (W.Curt.:Fr.) Lloyd (Aphyllophoromycetideae) Cooperatively Induces Tumor Necrosis Factor-α and Interleukin-6 with Lipopolysaccharide by Binding to Dectin-1
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 1&2
Mi Jeong Kim, Ha Won Kim, Se Young Choung, Dong Hee Lee, Byong Kak Kim, Su Kyoung Sung
Effect of Sasa veitchii Extract on Immunostimulating Activity of β-glucan (SCG) from Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Mia Yoshida, Naohito Ohno, Toshie H. Hida, Natsuko Akachi
Dectin-1 and GM-CSF on Immunomodulating Activities of Fungal 6-Branched 1,3-β-Glucans
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 2
Naohito Ohno, Toshie Harada
The Receptor for β-Glucan of Ganoderma lucidum (W.Curt.:Fr.) Lloyd in Immune Cells
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Ha Won Kim, Byong Kak Kim
The Anti-Inflammatory Effects of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Higher Basidiomycetes) in a Coculture System of 3T3-L1 Adipocytes and RAW264 Macrophages
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 7
Noriyasu Hirasawa, Kenji Ouchi, Koichiro Mori