Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i1.70
pages 61-68

Purification and Characterization of a Lectin Isolated from the Submerged Cultivated Mycelium of Grey Polypore Cerrena unicolor (Bull.) Murrill (Aphyllophoromycetideae)

Nona A. Mikiashvili
Institute of Evolution, University of Haifa, Israel; Iv. Javakhishvili State University of Tbilisi, Department of Biochemistry and Biotechnology, Georgia; Mushroom Biology & Fungal Biotechnology Laboratory, North Carolina A&T State University, USA
Vladimir I. Elisashvili
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia
Mulumebet Worku
The School of Agriculture and Environmental Sciences, North Carolina A & T State University, Greensboro, NC 27411, USA
Elene Davitashvili
Faculty of Natural Science, Iv. Javakhishvili State University, Tbilisi, Georgia
Omoanghe S. Isikhuemhen
Mushroom Biology and Fungal Biotechnology Laboratory, Department of Natural Resources and Environmental Design, College of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, Greensboro, North Carolina

ABSTRACT

Cerrena unicolor HAI 1095 lectin from vegetatively grown mycelium was isolated and characterized. Two N-acetyl-D-galactosamine- and one galactose-specific lectin fractions were purified from the mycelia of C. unicolor by a procedure consisting of ammonium sulfate precipitation, gel filtration on a Toyopearl HW-55F column, affinity chromatography and gel filtration on Ultrapac TSK G3000 SW. The C2 lectin fraction was effectively inhibited by N-acetyl-D-galactosamine (GalNAc), galactose (Gal), and lactose at minimal concentrations of 0.39, 0.78, and 1.56 mM, respectively. The hemagglutinating activity of the isolated lectin fraction C2 was stable between 4.0 and 7.5 mM of HCl and NaOH and at temperatures up to 50°C. Mg2+, Cu2+, Ba2+, and Fe3+ increased the hemagglutinating activity of the C2 lectin fraction. C. unicolor mycelium grown on malt extract agar medium accumulates high levels of lectin. The fungus produced two lectin fractions with N-acetyl-D-galactosamine specificity and one fraction with galactose specificity. This review reports on a study on lectin accumulation by C. unicolor. It provides further knowledge about new lectin properties and shows that C. unicolor submerged grown mycelium may be an important source of lectin with high yields.


Articles with similar content:

Comparative Study of Lectin Activity of Higher Basidiomycetes
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 1
Solomon P. Wasser, Nona A. Mikiashvili, Vladimir I. Elisashvili, Eviatar D. Nevo
Evaluation of Antibacterial Potential of Two Species of Genus Agaricus L: Fr. (Agaricomycetideae) from India
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 2
Inder S. Rana, Sardul S. Sandhu, Aarti Kanojiya
Medium Selection for Exopolysaccharide and Biomass Production in Submerged Cultures of Culinary-Medicinal Mushrooms from Turkey
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Mustafa Yamac, Meltem Kizilcik
Evaluation of Higher Basidiomycetes Mushroom Lectin Activity in Submerged and Solid-State Fermentation of Agro-Industrial Residues
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 2
Elene Davitashvili, Ekaterine Kapanadze, Eva Kachlishvili, Tamar Khardziani, Vladimir I. Elisashvili
Preparation of Culinary-Medicinal King Oyster Mushroom Pleurotus eryngii−Fermented Products with High Ergothioneine Content and Their Taste Quality
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 1
Jeng-Leun Mau, Ling-Yi Huang, Ching-Hsuan Tsai, Kung-Jui Ho, Shin-Yu Chen, Chih-Hung Liang