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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i3.100
pages 309-328

Medicinal Species from Genera Inonotus and Phellinus (Aphyllophoromycetideae): Cultural-Morphological Peculiarities, Growth Characteristics, and Qualitative Enzymatic Activity Tests

Daniel Tura
Aloha Medicinals Inc., Carson City, Nevada, USA
Ivan V. Zmitrovich
Laboratory of Systematics and Geography of the Fungi of the Komarov Botanical Institute of the Russian Academy of Sciences, Prof. Popova St. 2, St. Petersburg, 197376, Russia
Solomon P. Wasser
International Centre for Biotechnology and Biodiversity of Fungi Institute of Evolution and Faculty of Natural Sciences University of Haifa, Mt. Carmel, Haifa 31905, Israel
Eviatar D. Nevo
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, Institute of Evolution, University of Haifa, 199 Abba Khousi Ave., Mt. Carmel, Haifa 3498838, Israel

ABSTRACT

The morphological and cultural characteristics of vegetative mycelium of nine important medicinal species from genera Inonotus and Phellinus (Hymenochaetales) were studied. The species studied were Inonotus dryophilus, I. hispidus, I. levis, I. rheades, I. rickii, I. obliquus, I. tamaricis, Phellinus baumii, and Ph. linteus, represented by 14 strains. Data on mycelial growth of the above-mentioned fungi species on different types of nutrients, the macro- and micromorphological description of colonies grown on different media, and qualitative enzymatic activity tests on lignolytic enzymes are provided. It was shown that species Inonotus obliquus (3.57 mm/day), Ph. baumii (3.26 mm/day), and Ph. linteus (3.05 mm/day) demonstrated moderately slow growth on selected nutrients. By contrast, Inonotus levis recorded a rapid growth, with the highest growth rate value of all analyzed strains (8.63 mm/day). Most of examined species preferred wort agar, whereas I. rheades, I. rickii, and I. obliquus preferred potato-dextrose agar as the medium for optimal development. The least favorable nutrient for all taxa was corn agar. Analyses of morphological and microstructural peculiarities on different types of nutrients, with mycelial samples taken from different parts of the colony as advancing zone, marginal agar, and aerial mycelium, were conducted and illustrated for five species. As expected, the qualitative enzymatic activity tests confirmed a positive reaction to most of the analyzed strains. In addition, we observed that enzyme secretion is related to the medium type on which the colony is grown.