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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i3.90
pages 303-307

Specrophotometric Semi-Micro Determination of Ergosterol in Selected Medicinal Indian Phellinus Quél. (Aphyllophoromycetideae) Species

Prasad Y. Lamrood
Department of Botany, Ahmednagar College (affiliated with Savitribai Phule Pune University), Ahmednagar, Maharashtra, India

ABSTRACT

The present study reports the presence of ergosterol in 16 Indian medicinal Phellinus species for the first time. The samples were extracted at room temperature: first in alcoholic KOH, then in a mixture of distilled water and n-heptane. The upper n-heptane layer was pooled and analyzed between the spectrum of 200−300 nm. The ergosterol content was found to be the same in Ph. lloydii and Ph. minutiporus (0.26 mg−100g), Ph. sublinteus and Ph. orientalis (0.3 mg−100g), and Ph. coffeatoporus and Ph. aureobrunneus (0.12 mg−100g), respectively, whereas Ph. crocatus and Ph. torulosus contained 0.01 mg−100g ergosterol. In other Phellinus samples, the amount of ergosterol was relatively less. Furthermore, during spectrophotometric analysis, distinct peaks were observed in Ph. aureobrunneus, Ph. coffeatoporus, Ph. crocatus, Ph. griseoporus, Ph. torulosus, and Ph. linteus, as well as in Ph. lloydii, Ph. sublinteus, and Ph. minutiporus. However, the peaks were less distinct in seven samples such as Ph. badius, Ph. orientalis, Ph. pappinus, Ph. adamantinus, Ph. fastuosus, Ph. merrillii, and Ph. melanodermus.