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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i2.80
pages 177-183

Preliminary Studies on Mating and Improved Strain Selection in the Tropical Culinary-Medicinal Mushroom Lentinus squarrosulus Mont. (Agaricomycetideae)

Omoanghe S. Isikhuemhen
Mushroom Biology and Fungal Biotechnology Laboratory, Department of Natural Resources and Environmental Design, College of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, Greensboro, North Carolina
Clementina O. Adenipekun
Department of Botany and Miocrobiology, University of Ibadan, Ibadan, Nigeria
Elijah I. Ohimain
Mushroom Biology & Fungal Biotechnology Laboratory, North Carolina A&T State University, Greensboro, NC 27411, USA; and Biological Sciences Department, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria


Lentinus squarrosulus Mont. is an important tropical edible and medicinal mushroom distributed across Sub-Saharan Africa and most parts of Asia. Intrastock mating study was performed using single spore isolates (ssi) from a wild strain (MBFBL 201). Mycelia growth and morphology characterization indicated that milled corn straw agar (CSA) medium best supports mycelia growth compared to two other media: potato dextrose agar (PDA) and yeast peptone soluble starch agar (YPSS). The results from ssi pairings and analysis confirmed a tetrapolar mating system for L. squarrosulus. Further characterization of the resulting dikaryons indicated that the growth rate of the parent monokaryons does not affect the growth rate of the resulting dikaryon. Primordial formation in the resulting dikaryons with the fastest growth rate was 12 days after inoculation. Under similar conditions, MBFBL 201 and another wild strain, MBFBL 269, produced primordia after 28 and 32 days, respectively, indicating that intrastock breeding could be an effective method for obtaining improved strains of L. squarrosulus. Further characterization to test growth and performance in order to obtain high-yield strains for use in commercial cultivation is ongoing.

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