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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v4.i2.80
12 pages

Characterization of Somatic Hybrids with Compatible and Incompatible Species by Protoplast Fusion in Genera Pleurotus (Fr.) P. Karst. and Ganoderma P. Karst. by RAPD-PCR Analysis

Young Bok Yoo
Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, South Korea
Kang Hyo Lee
Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suweon 441-707, South Korea
Beom Gi Kim
Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, South Korea

ABSTRACT

A comparison of somatic hybrids with compatible and incompatible strains was made using random amplified polymorphic DNA (RAPD) analysis. The heterokaryons between compatible species, Pleurotus ostreatus and P. florida, showed the same level of variability and contained both parental RAPD bands. In contrast, most of the synkaryons between incompatible species such as P. cornucopiae + P. florida, P. ostreatus + Ganoderma applanatum, and P. florida + Ganoderma lucidum showed similarilarity to those of either parental bands or nonparental RAPD bands. Synkaryons can be classified into microgenome insertion type and macrogenome insertion type.


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