Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v18.i8.30
pages 671-680

Characterization of Cross-Flow Ultrafiltration Fractions from Maitake Medicinal Mushroom, Grifoia frondosa (Agaricomycetes), Reveals Distinct Cytotoxicity in Tumor Cells

Miriam Sari
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Reinhard Hambitzer
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Jan I. Lelley
Company for Applied Mycology and Environmental Studies Inc. (GAMU) in Krefeld, Germany
Katharina Toepler
Bio-Pharmaceutical Chemistry and Molecular Pharmacology, Technische Hochschule Koeln, Leverkusen, Germany
Nicole Teusch
Bio-Pharmaceutical Chemistry and Molecular Pharmacology, Technische Hochschule Koeln, Leverkusen, Germany
Anna Nickisch-Hartfiel
Department of Chemistry, Niederrhein University of Applied Sciences, Krefeld, Germany

ABSTRACT

β-glucans from Basidiomycetes like Grifola frondosa (the maitake mushroom) are well known for their health benefits. Polysaccharide preparations from medicinal mushrooms such as G. frondosa have been successfully tested in a vast number of studies. Many mushroom extracts have been developed and today are merchandized for use medicinally and commercially. Studies could show that, in particular, chemical structural features such as the molecular size of β-glucans significantly influence their bioactivity. Thus it is highly important to explore the composition and structural properties of β-glucans extracted from medicinal mushrooms and their effects on human tumor cell viability. Our study focuses on the molecular weight cutoff distribution of β-glucans in hot water−based extracts from maitake mushrooms. Cross-flow ultrafiltration was applied to obtain 5 fractions of different molecular size. β-glucan content was quantified using an enzyme-based test kit, specialized to 1,3-1,6-β-glucans. Here we show that only small amounts of β-glucans with a high molecular weight (>100 kDa) could be detected from an aqueous extract of G. frondosa. The main compounds encompass substances with a low molecular weight (<5 kDa), composing about 35% of the whole extract. In addition, tumor cell viability studies demonstrate significant cytotoxic potential in 2 different solid cancer cell types for the fraction with a high molecular weight (>100 kDa) and for 1 fraction with a low molecular weight (5−10 kDa). In summary, our experiments prove that cross-flow ultrafiltration serves as a quick and easy method for dividing crude aqueous mushroom extracts into different molecular-weight fractions that inhibit tumor cell viability in vitro.


Articles with similar content:

Antioxidant Activity of Edible Blushing Wood Mushroom, Agaricus sylvaticus Schaeff. (Agaricomycetideae) In Vitro
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 2
Audrey S. Naufal, Jorge L. Gennari, Sandro Percario, Marcelo S. Gennari
Cross-Flow Ultrafiltration Fractions of a Cold Aqueous Extract of the Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes), Exhibit Apoptosis in Tumor Cells
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 11
Miriam Sari, Nicole Teusch, Katharina Toepler, Anna Nickisch-Hartfiel, Reinhard Hambitzer
Evaluation of Anticholinesterase and Inflammation Inhibitory Activity of Medicinal Mushroom Phellinus pini (Basidiomycetes) Fruiting Bodies
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Tae Soo Lee, Jae-hyuk Choi, Jae Kwang Kim, Trung Kien Nguyen, Kyung Hoan Im
Antiproliferative Activities of Hot Water Extracts from Culinary-Medicinal Mushrooms, Ganoderma tsugae and Agrocybe cylindracea (Higher Basidiomycetes) on Cancer Cells
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Jeng-Leun Mau, Shu-Yao Tsai, Eric Icheng Lai, Rao-Chi Chien
Comparison of Antioxidant and Antiproliferation Activities of Polysaccharides from Eight Species of Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 3
Yangyang Yong, Yifan Gu, Zeliang Wang, Shizhu Zhang, Peiying Chen, Ling Lu