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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v14.i6.70
pages 593-602

Determination of Optimal Extraction Time and Temperature by Response Surface Methodology to Obtain High-Level Antioxidant Activity in Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.:Fr.) P. Kumm. (Higher Basidiomycetes)

Sze Mun Lim
Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia
Hip Seng Yim
Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, Kuala Lumpur; School of Food Science and Nutrition, Universiti Malaysia Sabah, Sabah

ABSTRACT

A central composite design of response surface methodology (RSM) was employed to optimize the extraction time (X1: 266.4−393.6 min) and temperature (X2: 42.9−57.1°C) of Pleurotus ostreatus aqueous extract with high antioxidant activities, namely DPPH radical-scavenging activity, ABTS radical cation inhibition, and ferric re-ducing/antioxidant power, as well as total phenolic content (TPC). Results showed that the data were adequately fitted into four second-order polynomial models developed by RSM. The extraction time and temperature were found to have significant quadratic effects on antioxidant activities and TPC. The optimal extraction time and temperature were 282.3 min and 42.9°C (DPPH), 393.6 min and 42.9°C (ABTS), 340.4 min and 49.8°C (FRAP), and 347.6 min, 49.7°C (TPC), with corresponding yields of 53.32%, 73.20%, 37.14 mM Fe2+ equivalents/100 g, and 826.33 mg gallic acid equivalents/100 g, respectively. These experimental data were close to their predicted values. The establishment of such a model provides a good experimental basis for employing RSM to optimize the extraction time and temperature for high antioxidant activities from P. ostreatus.


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