Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v18.i1.90
pages 75-81

Cytological Characterization of Anamorphic Fungus Lecanicillium pui and Its Relationship with Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes)

Wei Lei
Laboratory of Cardiovascular Diseases, Guangdong Medical College, Zhanjiang, China; Food and Health Engineering Research Center of State Education Ministry, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Guren Zhang
Food and Health Engineering Research Center of State Education Ministry, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Guangguo Wu
Food and Health Engineering Research Center of State Education Ministry, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Xin Liu
Food and Health Engineering Research Center of State Education Ministry, School of Life Sciences, Sun Yat-sen University, Guangzhou, China

ABSTRACT

Ophiocordyceps sinensis (syn. Cordyceps sinensis), one of the most valuable medicinal mushrooms, has great economic importance on the Tibetan Plateau. We isolated an anamorphic fungus Lecanicillium pui from natural O. sinensis specimens and found that the optimal temperature for its culture on potato dextrose agar media was 25°C. Cell ultrastructure in L. pui hyphae and spores was characterized by transmission electron microscopy, and it was observed that some primary organelles showed the typical fungal features. Five chemical elements were determined in this fungus and niobium was discovered for the first time even with trace amounts. A species-specific method, nested polymerase chain reaction, was established to investigate the colonization of this fungus. Thus, the extensive distribution of L. pui on O. sinensis, in the shape of hyphae or mycelia, suggested that it may have subtle and chronic effects on the growth of the O. sinensis teleomorphic stage. These findings provide a potential reference, in the view of microbial ecology, for the study on the occurrence and mechanism of development of O. sinensis.


Articles with similar content:

Genetic Resources and Mycelial Characteristics of Several Medicinal Polypore Mushrooms (Polyporales, Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 4
Alla V. Shnyreva, Susanna M. Badalyan, Alessandra Zambonelli, Mirco Iotti
Morphology, Growth Characteristics, and Genetic Variability of the Edible Medicinal Mushroom Flammulina velutipes (W. Curt.: Fr.) Singer Collections
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 3
Carmen Z. Sakeyan, Susanna M. Badalyan, Elizabeth Helmbrecht, Karen W. Hughes
Morphological, Physiological, and Growth Characteristics of Mycelia of Several Wood-Decaying Medicinal Mushrooms (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 4
Carmen Z. Sakeyan, Susanna M. Badalyan
HPLC Levels of Adenosine in Awheto—Indigenous New Zealand Cordyceps robertsii Hooker (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 1
Kevin Mitchell, Stephen Tauwhare
Fruiting Body Production of the Medicinal Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), in Artificial Medium
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 11
Li Cao, Richou Han, Yunshou Ye