Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v18.i1.60
pages 49-57

Activity of Extracts from Submerged Cultured Mycelium of Winter Mushroom, Flammulina velutipes (Agaricomycetes), on the Immune System In Vitro

Svetlana Kashina
Division de Ciencias Naturales y Exactas, Departamento de Biologia, Universidad de Guanajuato, Guanajuato, Guanajuato, México
Lerida Liss Flores Villavicencio
Division de Ciencias Naturales y Exactas, Departamento de Biologia, Universidad de Guanajuato, Guanajuato, Guanajuato, México
Silvio Zaina
Division de Ciencias de la Salud, Departamento de Ciencias Médicas, Universidad de Guanajuato, Leon, Guanajuato, México
Marco Balleza Ordaz
Division de Ingenierias, Departamento de Fisica Médica, Universidad de Guanajuato, Leon, Guanajuato, México
Gloria Barbosa Sabanero
Division de Ciencias de la Salud, Departamento de Ciencias Médicas, Universidad de Guanajuato, Leon, Guanajuato, México
Victor Tsutsumi Fujiyoshi
Centro de Investigacion y de Estudios Avanzados, Departamento de Infectomica y Patogénesis Molecular, Unidad Zacatenco, México, México
Myrna Sabanero Lopez
Division de Ciencias Naturales y Exactas, Departamento de Biologia, Universidad de Guanajuato, Guanajuato, Guanajuato, México

ABSTRACT

Extracts from submerged cultured mycelium of two strains of Flammulina velutipes, a popular culinary mushroom, were obtained by ultrasound and tested in vitro to determine their activity in innate immunity (monocytes/ macrophages). In addition, polyclonal antibodies against the extracts were produced. Both extracts have similar glycoproteins that contain mannose and glucose but have different glycoproteins with galactoseamine units. Two novel immunogenic glycoproteins with molecular weights of 32 and 25 kDa have been revealed. It is thought that these proteins are produced only by submerged cultured mycelium. Both extracts show immune-enhancing activity based on the significant modification of various parameters such as cytokine production, phagocytosis, and reactive oxygen species production.


Articles with similar content:

Dectin-1 and GM-CSF on Immunomodulating Activities of Fungal 6-Branched 1,3-β-Glucans
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 2
Naohito Ohno, Toshie Harada
Immunomodulating Activity of Bjerkandera sp.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Mark M. Shamtsyan, Valentina G. Konusova, Alexander M. Goloschev, Natalia V. Pigareva, Arina V. Korchmaryova
Characterization of Cross-Flow Ultrafiltration Fractions from Maitake Medicinal Mushroom, Grifoia frondosa (Agaricomycetes), Reveals Distinct Cytotoxicity in Tumor Cells
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 8
Miriam Sari, Nicole Teusch, Katharina Toepler, Jan I. Lelley, Anna Nickisch-Hartfiel, Reinhard Hambitzer
Antioxidative and Inhibitory Effects of the Fruiting Body of Black Lingzhi Mushroom, Amauroderma rugosum (Agaricomycetes), on LDL Oxidation and HMG-CoA Reductase Activity
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 9
Norhaniza Aminudin, Noorlidah Abdullah, Chan Kam Seng
Antioxidant and Immunomodulating Activities of Exo-and Endopolysaccharide Fractions from Submerged Mycelia Cultures of Culinary-Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 3
Karthik Marripudi, Cheang Khoo, Sundar Rao Koyyalamudi, Jong Pil Park, Jin Hee Kim, Sang-Chul Jeong, Chi-Hyun Song, J. Margaret Hughes, Trevor Bailey